Developmental Changes of Growth Hormone Mrna Abundance in Pituitary Tissue of Jinhua and Landrace Gilts

Abstract:

In this pepar we investigated the developmental patterns of expression of growth hormone (GH) gene in pituitary tissue in pigs of different breeds and their effects on the carcass fat contents. 3 Jinhua gilts and 3 Landrace gilts were sampled at 35, 80 and 125 days of age, respectively. Carcass fat contents were determined. Pituitary tissue was sampled and total RAN was extracted to determine GH mRNA expression levels by semi-quantitative RT-PCR. The results showed that the contents of carcass fat increased with growth and showed significant differences (P﹤0.05) between different age groups in the two breeds. Furthermore, carcass fat contents in Jinhua gilts were higher than that in Landrace gilts during growth (P﹤0.05). GH mRNA expression levels decreased with age and displayed breed differences. Jinhua gilts showed lower abundance of GH mRNA compared with Landrace gilts at 35, 80 and 125 days of age (P﹤0.05). In addition, GH mRNA expression level was negatively related to carcass fat content in Jinhua and Landrace gilts (r = -0.790 (P = 0.01), r = -0.755 (P = 0.02), respectively).

• Periodical: Advanced Materials Research (Volumes 343-344)
• Pages: 412-416
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.343-344.412
• Online since: September 2011
• Authors: Zhi Guo Miao, Guo Wang Li, Shi Zhu Wang, Xin Yao Chang, Hong Bing Xie, Chang Zhong Liu
• Keywords: Carcass Fat Content, Gene Expression, GH, Gilt, RT-PCR

References

[1] Y. Chilliard, Dietary fat and adipose tissue metabolism in ruminants, pigs and rodents: a review,. Journal of Dairy Science 1993, 76, 3897-3931.

DOI: 10.3168/jds.s0022-0302(93)77730-9

[2] B. Angelin, and M. Rudling, Growth hormone and hepatic lipoprotein metabolism,. Current Opinion in Lipidology 1994, 5, 160-165.

DOI: 10.1097/00041433-199405030-00002

[3] J. Oscarsson, M. Ottosson, K. Vikman-Adolfsson, F.F.S. Enerbäck, H. Lithell, and S. Edèn, GH but not IGF-I or insulin increases lipoprotein lipase activity in muscle tissues of hypophysectomised rats,. Journal of Endocrinology 1999, 160, 247-255.

DOI: 10.1677/joe.0.1600247

[4] L.J. Machlin, The effect of porcine growth hormone on growth and carcass composition of the pig,. Journal of Animal Science 1972, 35, 794-800.

DOI: 10.2527/jas1972.354794x

[5] R.G. Campbell, N.C. Steele, T.J. Caperna, J.P. Mcmurtry, M.B. Soloman, and A.D. Mitchell, Interrelationships between energy intake and endogenous porcine growth hormone administration on the performance, body composition and protein and energy metabolism of growing pigs weighing 25 to 55 kilograms live weight,. Journal of Animal Science 1988, 66, 1643-1655.

DOI: 10.2527/jas1988.6671643x

[6] S. Kousteni, F. Tura-Kockar and D.P. Ramji, Sequence and expression analysis of a novel Xenpus Laevis cDNA that encodes a protein similar to bacterial and chloroplast ribosomal protein L24,. Gene. 1999, 235: 13-18.

DOI: 10.1016/s0378-1119(99)00221-8

[7] H.J. Mersmann, J. M. Houk, G. Phinney and M.C. Underwood, Effect of diet and weaning age on in vitro lipogenesis in young swine,. Journal of Nutrition. 1973, 103: 821-828.

DOI: 10.1093/jn/103.6.821

[8] X.C. Yan, Y.Z. Wang and Z.R. Xu, Regulation of fatty acid synthase (FAS) gene expression in animals,. Acta Zoonutrimenta Sinica (In Chinese). 2002, 14: 1-4.

[9] J.R. Wanger, A.P. Schinckel, W. Chen, J.C. Forrest and B.L. Coe, Analysis of body composition changes of swine during growth and development,. Journal of Animal Science. 1999, 77: 1442-1466.

DOI: 10.2527/1999.7761442x

[10] D. D'Souza, D. Pethick, F. Dunshea, D. Suster, J. Pluske and B. Mullan, The pattern of fat and lean muscle tissue deposition differs in the different pork primal cuts of female pigs during the finisher growth phase,. Livestock Production Science. 2004, 91: 1-8.

DOI: 10.1016/j.livprodsci.2004.04.005

[11] D. B Anderson and R.G. Kauffman, Cellular and enzymatic changes in porcine adipose tissue during growth,. Journal of Lipid Research. 1973, 14: 160-168.

DOI: 10.1016/s0022-2275(20)36903-0

[12] C. Brook, G.P.C. Hindmarsh and R. Stanhope, Growth and growth hormone secretion,. Journal of Endocrinology 1988, 119, 179-184.

DOI: 10.1677/joe.0.1190179

[13] J.R. Florini, D.Z. Ewton and S.A. Coolican, Growth hormone and the insulin-like growth factor system in myogenesis,. Endocrine Reviews 1996, 17, 481-517.

DOI: 10.1210/edrv-17-5-481

[14] T. Etherton, The biology of somatotropin in adipose tissue growth and nutrient partitioning,. Journal of Nutrition 2000, 130, 2623-2625.

DOI: 10.1093/jn/130.11.2623

[15] I. Louveau and F. Gondret, Relation of development and metabolism of adipose tissue by growth hormone and the insulin-like growth factor system,. Domestic Animal Endocrinology. 2004, 27, 241-255.

DOI: 10.1016/j.domaniend.2004.06.004