Phospholipase D-Mediated Transphosphatidylation in Subcritical 1, 1, 1, 2-Tetrafluoroethane
Phospholipase D-mediated transphosphatidylation to phosphatidylserine from phosphatidylcholine was successfully performed using subcritical 1,1,1, 2-tetrafluoroethane (R134a) as reaction medium. Phospholipase D from Stretomyces sp was found to be active in subcritical R134a at 30-50°C and 2-8MPa; Higher velocity and conversion were observed with respect to those attained in conventional organic solvents. A maximum conversion of 84.88% was attained at 6 MPa, 40°C, after 240 minutes with 9.6 units phospholipase D.
H. Zhang et al., "Phospholipase D-Mediated Transphosphatidylation in Subcritical 1, 1, 1, 2-Tetrafluoroethane", Advanced Materials Research, Vols. 343-344, pp. 743-747, 2012