Phospholipase D-Mediated Transphosphatidylation in Subcritical 1, 1, 1, 2-Tetrafluoroethane

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Phospholipase D-mediated transphosphatidylation to phosphatidylserine from phosphatidylcholine was successfully performed using subcritical 1,1,1, 2-tetrafluoroethane (R134a) as reaction medium. Phospholipase D from Stretomyces sp was found to be active in subcritical R134a at 30-50°C and 2-8MPa; Higher velocity and conversion were observed with respect to those attained in conventional organic solvents. A maximum conversion of 84.88% was attained at 6 MPa, 40°C, after 240 minutes with 9.6 units phospholipase D.

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Advanced Materials Research (Volumes 343-344)

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743-747

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September 2011

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© 2012 Trans Tech Publications Ltd. All Rights Reserved

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[1] T.H. Crook, J. Tinklenberg, J. Yesavage, et al., Neurology. 41 (1991) 644-649.

Google Scholar

[2] T.H. Crook, W. Petrie,C. Wells, et al., Psychopharmacology Bulletin. 28 (1992) 61-66.

Google Scholar

[3] Comfuris. P, Bvers. E. M, Zwaal. R.F. A, J. Lipid Res. 31(1990) 1719-1721.

Google Scholar

[4] Juneja. L. R, Kazuoka. T, Goto. N, et al., Biochim. Biophys. Acta. 1003 (1989a) 277-283.

Google Scholar

[5] Kamata. M, Hara. S, Totani. Y, J. Jpn. Oil Chem. Soc. 47(1998) 51-56.

Google Scholar

[6] T.W. Randolph, H.W. Blanch, J.W. Prausnitz, et al., Bitechnol. Lett. 7(1985) 3215-3218.

Google Scholar

[7] D.A. Hammond, M. Karel, A.M. Klibanov, Appl. Biochem. Biotechnol. 11(1985) 393-400.

Google Scholar

[8] K. Nakamura,Y. Min Chi, Y. Yamada, et al., Chem. Eng. Commun. 45 (1986) 207-212.

Google Scholar

[9] T. Ikariya, Y. Kayaki, Catal. Surv. Jpn. 4 (2000) 39-50.

Google Scholar

[10] O. J. Catchpole, K. Proells, Ind. Eng. Chem. Res. 40 (2001) 965-972.

Google Scholar

[11] Wilde. P. F, New Solvent for Extraction, Perfum. Flavor. 1 9(1994) 25-28.

Google Scholar

[12] Wilde. P. F, Fragrance Extraction. European Patent Application, EP616821 A1, (1994).

Google Scholar

[13] K. Jackson, L. E. Bowman, J. L. Fulton, Anal. Chem. 67 (1995) 2368-2372.

Google Scholar

[14] Lopez-Belmonte M. T, Alca´ntara A. R, Sinisterra J. V, J. Org . Che. 62 (1997) 1831– 1840.

Google Scholar

[15] Sakurai T, Malgolin A. L, Russell A. J, Klibanov A.M. J . Am. Chem . Soc. 113(1991) 695– 696.

Google Scholar

[16] Terradas. F, Teston-Henry. M, Fitzpatric. PA, et al., J . Am. Chem. Soc . 115(1993) 390–396.

Google Scholar

[17] M. Hosokawa, T. Shimatani, T. Kanada, et al., J. Agric. Food. Chem. 4 8(2000) 4550-4554.

Google Scholar

[18] C. Virto,I. Svensson, P. Adlercreutz, Chem. Phys. Lip. 106 (2000) 41.

Google Scholar