Phospholipase D-Mediated Transphosphatidylation in Subcritical 1, 1, 1, 2-Tetrafluoroethane

Abstract:

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Phospholipase D-mediated transphosphatidylation to phosphatidylserine from phosphatidylcholine was successfully performed using subcritical 1,1,1, 2-tetrafluoroethane (R134a) as reaction medium. Phospholipase D from Stretomyces sp was found to be active in subcritical R134a at 30-50°C and 2-8MPa; Higher velocity and conversion were observed with respect to those attained in conventional organic solvents. A maximum conversion of 84.88% was attained at 6 MPa, 40°C, after 240 minutes with 9.6 units phospholipase D.

Info:

Periodical:

Advanced Materials Research (Volumes 343-344)

Edited by:

David Wang

Pages:

743-747

DOI:

10.4028/www.scientific.net/AMR.343-344.743

Citation:

H. Zhang et al., "Phospholipase D-Mediated Transphosphatidylation in Subcritical 1, 1, 1, 2-Tetrafluoroethane", Advanced Materials Research, Vols. 343-344, pp. 743-747, 2012

Online since:

September 2011

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Price:

$35.00

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