Ultrasonic-Assisted Dialysis Coupled with CE-ICP-OES for Determination of Calcium Species in the Dialysate of Human Blood

Abstract:

The analytical method for calcium species in human blood was studied using ultrasonic-assisted dialysis coupled with capillary electrophoreisi (CE) inductively coupled plasma optical emission spectrometry (ICP-OES). The optimal ultrasonic dialysis conditions were 72 W power, 60 min extraction time and 70 μL volume of blood sample. Ultrasonic-assisted dialysis improves significantly the dialysis efficiecy by comparing with balance dialysis. The optimized conditions of separation and detection (a 120 cm length×100 μm i.d. fused-silica capillary, 20 kV separation voltage, 30 mmol/L Tris-HCl with pH7.4 buffer solution) for calcium species were achieved by CE-ICP-OES. The forms of calcium in the dialysate of human blood have five different species. The concentration of free Ca²⁺ in the dialysate was 0.7 mg/L. The hyphenated technique is simple and convenience, which is fit for the separation and analysis of metal-containing biological samples.

• Periodical: Advanced Materials Research (Volumes 343-344)
• Pages: 769-773
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.343-344.769
• Online since: September 2011
• Authors: Bi Yang Deng, Xiang Shu Xu, Ying Zi Wang, Ping Chuan Zhu
• Keywords: Calcium, Capillary Electrophoresis, Inductively Coupling Plasma Optical Emission Spectrometry, Speciation Analysis, Ultrasonic Dialysis

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