Induction of Metallothionein by Roxarsone in Earthworm Eisenia Andrei

Abstract:

Metallothionein (MT) is one kind of proteins induced in organism under the stress of heavy metal. It plays a great pole in detoxification and tolerance of heavy metal. In this study, according to the rich conservative sequence in MT, we designed corresponding primer, then cloned MT mRNA gene induced by As in earthworm E. andrei and got its nucleotide sequence. The results proved the presence of MT in Eisenia andrei, and showed that roxarsone could induce the expression of arsenic MT gene.

• Periodical: Advanced Materials Research (Volumes 343-344)
• Pages: 781-786
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.343-344.781
• Online since: September 2011
• Authors: Yin Sheng Li, Xiu Hong Wang, Jiang Ping Qiu, Jing Sun, Ji Bao Jiang
• Keywords: Gene, Induction, Metallothionein, mRNA, Roxarsone, RT-PCR

References

[1] T. C. Hancock, C. V. Miller, J. M. Denver. Fate and Transport of Arsenical Feed Amendments in Chesapeake Bay Watersheds, Society of Environmental Toxicology and Chemistry 21st Annual Meeting, November 12-16 Nashville, Tenn. (2000).

[2] B. L. Brown, A. D. Slaughter, M. E. Schreiber, Controls on roxarsone transport in agricultural watersheds, Appl. Geochem., vol. 20, pp.123-133, (2005).

DOI: 10.1016/j.apgeochem.2004.06.001

[3] J. R. Garbarino, A. J. Bednar, D. W. Rutherford, R. S. Beyer, R. L. Wershaw, 2003. Environmental fate of roxarsone in poultry litter. I. Degradation of roxarsone during composting, Environ. Sci. Technol., vol. 37, pp.1509-1514, (2003).

DOI: 10.1021/es026219q

[4] A. M. A. Rida, Les vers de terre et l'environnement, La Recherche, vol. 25, pp.260-267, (1994).

[5] M. B. Bouché., J. P. Qiu. Contributions concretes des lombriciens aux études environnementales, Docpédozool. intégrol., vol. 3, pp.225-252, (1998).

[6] L. D. Lehman-McKeeman, C. D. Klaassen. Induction of metallothionein- I and metallothionein-II in rats by cadmium and zinc, Toxicol. Appl. Pharm., vol. 88, p.195–202, (1987).

DOI: 10.1016/0041-008x(87)90005-6

[7] A. Furst, Q. Nguyen. Cadmium induced metallothionein in earthworms (Lumbricus terrestris). Biological and Trace Element Research, Biol. Trace Elem. Res., vol. 21, p.81–85, (1989).

DOI: 10.1007/bf02917239

[8] Y. S. Li, Z. L. Zeng, Z. L. Chen, P. Robin, M. Hassouna, J. P. Qiu, Effects of roxarsone on CYP4501A and CYP4502B in Eisenia andrei, Agrochimica, vol. 51, pp.8-18, (2007).

[9] Y. Li, Z. Zeng, Z. Chen, J. P. Qiu. Accumulation and elimination of arsenic in earthworms under stress of roxarsone contamination, J. Tongji University (Natural Science), vol. 36, pp.212-217, (2008).

[10] Y. S. Li, Y. Q. Xiao, J. P. Qiu, Y.Q. Dai, P. Robin, Continuous village sewage treatment by vermifiltration and activated sludge process, Water Sci. Technol., vol. 60, pp.3001-3010, (2009).

DOI: 10.2166/wst.2009.715

[11] Y. S. Li, P. Robin, D. Cluzeau, M. Bouche, J. P. Qiu, A. Laplanche, M. Hassouna, et al., Vermifiltration as a stage in reuse of swine wastewater: Monitoring methodology on an experimental farm, Ecol. Eng., vol. 32, pp.301-309, (2008).

DOI: 10.1016/j.ecoleng.2007.11.010

[12] A. K. Covey, D. J. Furbish, K. S. Savage. Earthworms as agents for arsenic transport and transformation in roxarsone-impacted soil mesocosms: A mu XANES and modeling study, Geoderma., vol. 156, pp.99-111, (2010).

DOI: 10.1016/j.geoderma.2010.02.004

[13] S. R. Stuirzenbaum, P. Kille, A. J. Morgan. Morgan Heavy metal-induced molecular responses in the earthworm, lumbricus rubellus genetic fingerprinting by directed different display, Appl. Soil Ecol., vol. 9, pp.495-500, (1998).

DOI: 10.1016/s0929-1393(98)00111-5

[14] M. B. Bouché. Lesversdeterre, La Recherche, vol. 156, pp.796-804, (1984).

[15] C. Gruber, S. Sturzenbaum, P. Gehrig, R. Sack, P. Hunziker, B. Berger, R. Dallinger,. Isolation and characterization of a self-sufficient one-domain protein (Cd)-Metallothionein from Eisenia foetida, Eur. J. Biochem., vol. 267, pp.573-582, (2000).

DOI: 10.1046/j.1432-1327.2000.01035.x