Determination of Erythromycin and Haloperidol in Human Urine by Capillary Electrophoresis Online Coupled with Electrochemiluminescence Detection
This work developed a simple and sensitive method for the simultaneous determination of erythromycin (Ery) and haloperidol (Hal) in human urine by capillary electrophoresis with eletrochemiluminescence detection. Under optimized conditions, such as detection potential at 1.25 V, electrokinetic injection at 10 kV for 6 s, separation voltage at 10 kV, 15 mmol/L separation buffer with pH 6.5, 5 mmol/L Ru(bpy)32+ and 50 mmol/L phosphate buffer with pH 8.0 in the ECL cell, the linear concentration ranges for Ery and Hal were from 0.005 to 0.2 μg/mL and from 0.15 to 6.0 μg/mL, respectively. The detection limits (3σ) for Ery and Hal were 0.002 and 0.06 μg/mL, respectively. When the method was applied to determine Ery and Hal in human urine, the recoveries were 96.5% and 95.1% on an average, respectively.
B. Y. Deng et al., "Determination of Erythromycin and Haloperidol in Human Urine by Capillary Electrophoresis Online Coupled with Electrochemiluminescence Detection", Advanced Materials Research, Vols. 343-344, pp. 764-768, 2012