Construction and Vaccine Studies on a Pseudorabies Virus with Deletions in Glycoprotein I and Glycoprotein E Genes

Abstract:

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A pseudorabies virus (PRV) mutant with deletions in genes for glycoprotein I (gI) and glycoprotein E (gE) was constructed and evaluated as a candidate vaccine strain for its protective by virulent wildtype (wt) PRV Ea strain challenge in pigs. Transfer plasmid pIESE was constructed and co-transfected with PRV genome into IBRS-2 cells to generate a recombinant mutant PRV Ea gI-/gE- virus. The recombinant virus was confirmed by Southern blotting and indirect immunofluorescence assay (IFA). In animal experiments, the immunogenicity was tested by PRV-enzyme-linked immunosorbent assay (ELISA) and PRV neutralizing assay. PRV Ea gI-/gE- elicited significant humoral immune responses to wt PRV Ea, and PRV gI-/gE- immunization protected pigs against a lethal challenge by virulent PRV Ea strain. These results suggested that the recombinant PRV Ea gI-/gE- might be considered as a potential candidate vaccine against PRV.

Info:

Periodical:

Advanced Materials Research (Volumes 343-344)

Edited by:

David Wang

Pages:

868-874

DOI:

10.4028/www.scientific.net/AMR.343-344.868

Citation:

S. L. Zhang et al., "Construction and Vaccine Studies on a Pseudorabies Virus with Deletions in Glycoprotein I and Glycoprotein E Genes", Advanced Materials Research, Vols. 343-344, pp. 868-874, 2012

Online since:

September 2011

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Price:

$35.00

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