Construction and Vaccine Studies on a Pseudorabies Virus with Deletions in Glycoprotein I and Glycoprotein E Genes

Abstract:

A pseudorabies virus (PRV) mutant with deletions in genes for glycoprotein I (gI) and glycoprotein E (gE) was constructed and evaluated as a candidate vaccine strain for its protective by virulent wildtype (wt) PRV Ea strain challenge in pigs. Transfer plasmid pIESE was constructed and co-transfected with PRV genome into IBRS-2 cells to generate a recombinant mutant PRV Ea gI^-/gE^- virus. The recombinant virus was confirmed by Southern blotting and indirect immunofluorescence assay (IFA). In animal experiments, the immunogenicity was tested by PRV-enzyme-linked immunosorbent assay (ELISA) and PRV neutralizing assay. PRV Ea gI^-/gE^- elicited significant humoral immune responses to wt PRV Ea, and PRV gI^-/gE^- immunization protected pigs against a lethal challenge by virulent PRV Ea strain. These results suggested that the recombinant PRV Ea gI^-/gE^- might be considered as a potential candidate vaccine against PRV.

• Periodical: Advanced Materials Research (Volumes 343-344)
• Pages: 868-874
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.343-344.868
• Online since: September 2011
• Authors: Song Lin Zhang, Mei Lin Jin, Huan Chun Chen
• Keywords: Deletion Mutant, Homologous Recombination, Pseudorabies Virus (PRV), Vaccine

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