Characteristics of Lysozyme Adsorption on Magnetic Fe₃O₄/Chitosan Nanoparticles at Fixed pH

Abstract:

Magnetic Fe₃O₄/chitosan nanoparticles were synthesized for lysozyme separation from solution. The adsorption of lysozyme was investigated on magnetic Fe₃O₄/chitosan nanoparticles at fixed pH 6.0, because the enzymatic activity of lysozyme reaches its maximum in this condition. The influence of initial lysozyme concentration, temperature and contact time on lysozyme adsorption was studied. The results of lysozyme adsorption indicated that the adsorption isotherm fitted Sips model well. The maximum adsorption capacity was 144.11mg/g at 310 K. The thermodynamic parameters, ΔG⁰, ΔH⁰ and ΔS⁰, illustrated that the adsorption of lysozyme was endothermic and spontaneous process.

• Periodical: Advanced Materials Research (Volumes 343-344)
• Pages: 909-913
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.343-344.909
• Online since: September 2011
• Authors: Yan Lan Lin, Ren Wang, Wei Ma, Zi Hong Cheng
• Keywords: Adsorption, Fixed pH, Lysozyme, Magnetic Fe3O4/Chitosan

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