Analysis for Effect of Dimehypo on Proteolytic Enzyme Activity in the Midgut of the Spider Pardosa pseudoannulata with a Piezoelectric Quartz Crystal Impedance

Abstract:

A piezoelectric quartz crystal impedance technique has been applied for in situ monitoring of the whole process of the proteolytic enzyme-catalyzed hydrolyzation of casein and the effect of pesticide dimehypo (formula: C5H11NNa2O6S4) on the proteolytic enzyme activity in the midgut of the spider Pardosa pseudoannulata, based on its real-time reponses to the density-viscosity change of the tested solution due to the hydrolyzation occurring on the casein molecules. The results indicated that the hydrolyzation was significantly increased in the presence of low-dose dimehypo, where significantly inhibited in the presence of high-dose dimehypo, and a correlation was found precisely between the enzyme activity reflected by the final frequency shift after the hydrolyzation and the concentration of dimehypo, with a significant frequency response observed even at a 2.88 x 10-4 volume ratio of dimehypo to distilled water. The present work may have presented a novel method feasible for study on the mechanism of excitability of spiders under low-dose dimehypo pesticide and quick detection of proteolytic enzyme activity.

• Periodical: Advanced Materials Research (Volume 345)
• Pages: 387-392
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.345.387
• Online since: September 2011
• Authors: Wen Hui Fu, Jun Fang, Zhi Wang
• Keywords: Dimehypo, Pardosa pseudoannulata, Piezoelectric Quartz Crystal Impedance, Proteolytic Enzyme Activity

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