Effect of the Venom of the Scorpion Heterometrus liangi on the Expression of P21 and Caspase-3 Gene in Human Kyse-510 Cell

K.F. Xiao; J. Zhou; Zhi Wang; Wen Hui Fu; Xiang Yang Lu

doi:10.4028/www.scientific.net/AMR.345.399

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HomeAdvanced Materials ResearchAdvanced Materials Research Vol. 345Effect of the Venom of the Scorpion Heterometrus...

Effect of the Venom of the Scorpion Heterometrus liangi on the Expression of P21 and Caspase-3 Gene in Human Kyse-510 Cell

Abstract:

This paper focuses on the effect of the venom of the scorpion Heterometrus liangi on the proliferation of human KYSE-510 cell line and the related molecular mechanism. Marked morpho-logical changes in KYSE-510 cells after treatment with scorpion venom were observed using scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Cell appopsis were deternined using flow cytometry. moreover, RT-PCR and Western blot analysis was used to research the different level of p21 and caspase-3 expression. These results suggest that scorpin venom at concentrations of 50–100 ug/mL caused dose- dependent inhibition of KYSE-510 cell proliferation. The ratio of apoptosis and necrosis increased. The effect of venom treated in KYSE-510 cells in p21 gene expression was reinforced, but the caspase-3 gene expression has indistinctive effect. The activity of p21 and caspase-3 protein expression were upregulated after scorpion venom treatment. scorpion venom is to activate the expressions of p21 and caspase-3 protein to cause selective cell apoptosis.

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Periodical:

Advanced Materials Research (Volume 345)

Pages:

399-404

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.345.399

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Online since:

September 2011

Authors:

K.F. Xiao, J. Zhou, Zhi Wang, Wen Hui Fu, Xiang Yang Lu

Keywords:

Cell Proliferation, Cytotoxicity, Gene Expression, Heterometrus Liangi Venom, KYSE-510 Cells

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