The in Silicon Cloning ILF2 Gene and Prediction of ILF2 Protein Structure in Sheep

Abstract:

In silicon cloning, we obtained ILF2 gene by using human ILF2 gene sequence (NM_004515) to be probe. Sequence analysis showed that the in silicon cloned cDNA was 1662 base pairs long with an open reading frame (ORF) containing 1173 nucleotides encoding a protein of 390 amino acids. 5’-untranslated region (UTR) was 74 bp, and 3’-UTR was 413 bp. A comparison of the sheep ILF2 with cow, horse, human, mouse, xenopus and zebra fish ILF2 amino acids had 96%, 91%, 91%, 81%, 61%, and 54% identity. The PI was 5.19, and molecular weight of the deduced protein was 43 050.12 Da. The pig ILF2 contained a RGG-rich single-stranded RNA-binding domain and a DZF zinc-finger nucleic acid binding domain. This study laid a foundation for further analysis of structure, expression and regulation of ILF2 gene in sheep.

• Periodical: Advanced Materials Research (Volume 345)
• Pages: 423-428
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.345.423
• Online since: September 2011
• Authors: Ying Ning Sun, Yu Zhao, Wei Yu Wang
• Keywords: Bioinformatics, Expressed Sequence Tag (EST), In Silicon Cloning, Interleukin Enhancer Binding Factor 2 (ILF2), Open Reading Frame (ORF)

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