Molecular Cloning and Characterization Analysis of Low CO2–Inducible Protein Gene from Dunaliella parva
Microalgae could modulate their photosynthesis to acclimate to CO2-limiting stress through inducing a carbon-concentrating mechanism (CCM) which included carbonic anhydrases and inorganic carbon (Ci) transporters. However, Ci-speciﬁc transporters have not been well described in algae so far. The former studies indicated that low CO2-inducible protein (Lci) played a signiﬁcant role in the CCM, could increase Ci uptake and intracellular Ci accumulation under low CO2 conditions. The full-length cDNA encoding Lci was obtained from oleaginous microalgae Dunaliella parva, which include 1302 bp open reading frame (ORF), 255 bp 5′-untranslated sequence and 447 bp 3′-untranslated sequence. Similarity analysis revealed that the highest identity was found among Dunaliella parva, Chlamydomonas reinhardtii and Volvox carteri. The Dunaliella parva Lci also showed wide similarity with other species.
Weiguo Pan, Jianxing Ren and Yongguang Li
C. H. Shang et al., "Molecular Cloning and Characterization Analysis of Low CO2–Inducible Protein Gene from Dunaliella parva", Advanced Materials Research, Vols. 347-353, pp. 2705-2708, 2012