Molecular Cloning and Characterization Analysis of Low CO2–Inducible Protein Gene from Dunaliella parva

Chang Hua Shang; Shun Ni Zhu; Zhen Hong Yuan; Zhong Ming Wang

doi:10.4028/www.scientific.net/AMR.347-353.2705

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 347-353Molecular Cloning and Characterization Analysis of...

Molecular Cloning and Characterization Analysis of Low CO₂–Inducible Protein Gene from Dunaliella parva

Abstract:

Microalgae could modulate their photosynthesis to acclimate to CO2-limiting stress through inducing a carbon-concentrating mechanism (CCM) which included carbonic anhydrases and inorganic carbon (Ci) transporters. However, Ci-speciﬁc transporters have not been well described in algae so far. The former studies indicated that low CO2-inducible protein (Lci) played a signiﬁcant role in the CCM, could increase Ci uptake and intracellular Ci accumulation under low CO2 conditions. The full-length cDNA encoding Lci was obtained from oleaginous microalgae Dunaliella parva, which include 1302 bp open reading frame (ORF), 255 bp 5′-untranslated sequence and 447 bp 3′-untranslated sequence. Similarity analysis revealed that the highest identity was found among Dunaliella parva, Chlamydomonas reinhardtii and Volvox carteri. The Dunaliella parva Lci also showed wide similarity with other species.

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Periodical:

Advanced Materials Research (Volumes 347-353)

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2705-2708

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.347-353.2705

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Online since:

October 2011

Authors:

Chang Hua Shang, Shun Ni Zhu, Zhen Hong Yuan, Zhong Ming Wang

Keywords:

Dunaliella Parva, Low CO₂-Inducible Protein, Molecular Cloning, Sequence Analysis

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