Applications of Reversed-Phase High-Performance Liquid Chromatoraphy in Strains Identification of the Bean Sprout

Yong Zuo; Shuai Ju; Yang Li; Hui Xie; Li Ping Liu; Fang Min Wei; An Guo Liu

doi:10.4028/www.scientific.net/AMR.347-353.354

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 347-353Applications of Reversed-Phase High-Performance...

Applications of Reversed-Phase High-Performance Liquid Chromatoraphy in Strains Identification of the Bean Sprout

Abstract:

Lactic acid which was fermented by bean sprout was determined and the lactobacillus in bean sprout was identified by High-performance Liquid Chromatoraphy(HPLC). The optimal HPLC chromatographic conditions were determined as follows:Agilent XDB-C18 chromatography column with UV detection at 210nm.The mobile phase was Phosphate buffer solution（pH=2.3）:methanol:acetonitrile（95%:2%:3%）.The flow rate was 0.5ml/min.This method is accurate,Speedily and reproducible. This method can identify the lactobacillus, and gives a qualitative and quantitative analysis of the lactic acid which was the metabolites of Yibin bean sprout.

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Periodical:

Advanced Materials Research (Volumes 347-353)

Pages:

354-359

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.347-353.354

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Online since:

October 2011

Authors:

Yong Zuo, Shuai Ju, Yang Li, Hui Xie, Li Ping Liu, Fang Min Wei, An Guo Liu

Keywords:

Identifification, Lactic Acid Bacteria (LAB), RP-HPLC, Separation, Yibin Bean Sprout

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