Applications of Reversed-Phase High-Performance Liquid Chromatoraphy in Strains Identification of the Bean Sprout
Lactic acid which was fermented by bean sprout was determined and the lactobacillus in bean sprout was identified by High-performance Liquid Chromatoraphy(HPLC). The optimal HPLC chromatographic conditions were determined as follows:Agilent XDB-C18 chromatography column with UV detection at 210nm.The mobile phase was Phosphate buffer solution（pH=2.3）:methanol:acetonitrile（95%:2%:3%）.The flow rate was 0.5ml/min.This method is accurate,Speedily and reproducible. This method can identify the lactobacillus, and gives a qualitative and quantitative analysis of the lactic acid which was the metabolites of Yibin bean sprout.
Weiguo Pan, Jianxing Ren and Yongguang Li
Y. Zuo et al., "Applications of Reversed-Phase High-Performance Liquid Chromatoraphy in Strains Identification of the Bean Sprout", Advanced Materials Research, Vols. 347-353, pp. 354-359, 2012