Chromosome Aberrations Induced by 5-Azacytine in the Root Tips of Wheat Carrying the Gametocidal Chromosome 3C

Abstract:

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When the gametocidal chromosome 3C from Aegilops triuncialis was introduced into common wheat, it was preferentially transmitted and it had severe Gc action in Chinese Spring (CS). In this study, we used the root tips of Chinese Spring and Chinese Spring carrying the gametocidal chromosome 3C to analyze the relationship between gametocidal action and DNA methylation. They were treated with the hypomethylation agent 5-azacytine (5-AC) in 100µM concentration. In the root tips of Chinese Spring we observed abnormal division at a low frequency, which less than 1%. In contrast, in Chinese Spring-Gametocidal chromosome 3C disomic addition line a mass of chromosome aberrations were observed and the major form was chromosome breakage. This suggested that during early seed development chromosome breakage induced by the gametocidal chromosome 3C was repressed by DNA methylation. In addition, after 5-AC treatment the amount of chromosome breakage might be relative to Gc action.

Info:

Periodical:

Advanced Materials Research (Volumes 356-360)

Edited by:

Hexing Li, Qunjie Xu and Daquan Zhang

Pages:

228-231

DOI:

10.4028/www.scientific.net/AMR.356-360.228

Citation:

C. H. Guo et al., "Chromosome Aberrations Induced by 5-Azacytine in the Root Tips of Wheat Carrying the Gametocidal Chromosome 3C", Advanced Materials Research, Vols. 356-360, pp. 228-231, 2012

Online since:

October 2011

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$35.00

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