Study on Chromosome Aberrations of Crosses Between 3E Disomic Addition Line and 2C Addition Line
Thinopyrum elongatum, a wild relative of wheat, can be used as an excellent source of disease and abiotic stresses resistance and carries several useful traits, which have been transferred into wheat by interspecific hybridization. The most effective method of transferring the profitable genes of Th. elongatum into wheat is to induce chromosome recombination by the gametocidal system. In this study, cross, backcross and selfcross were performed between Chinese Spring-Th. elongatum 3E disomic addition line and Chinese Spring-Ae. cylindrica gametocidal chromosome 2C disomic addition line, and the gametocidal chromosome would induce chromosome breakages and aberrations. The chromosome counts of somatic cell and the observation of PMCs metaphase were performed in F2, BC1F1 and F1, F2 plants, respectively, the results showed that the gametocidal chromosome was functioning during the formation of gametes. The gametocidal chromosome 2C induced segregation for spike type in F2 and BC1F1 plants. Thus, the gametocidal chromosome 2C was demonstrated to be effective on transferring Th. elongatum 3E chromosome into wheat.
Hexing Li, Qunjie Xu and Daquan Zhang
C. H. Guo et al., "Study on Chromosome Aberrations of Crosses Between 3E Disomic Addition Line and 2C Addition Line", Advanced Materials Research, Vols. 356-360, pp. 236-239, 2012