Water Storage and Controlled-release Film (WSCF) is a new kind of polymer composite with the function of releasing water slowly in a given period. Micro-organism culture was carried out to study the process of film’s degradation. The results showed that the degradation rate of WSCF added with fungi suspension was increased by 0.6% more than that of WSCF without fungi suspension. The degradation rate of WSCF added with soil suspension was increased by about 1% more than the film without soil suspension. Compared different culture mediums, the order of degradation rates of WSCF was: bacteria medium > actinomyces medium > fungi medium. The degradation rate of WSCF with field soil suspension was the highest one. The degradation rate of WSCF was higher than ordinary plastic film, increased by 3-5%. The results of tests under liquid culture indicated that mixed bacterium were helpful to the degradation of WSCF, the degradation rate of WSCF under liquid culture was higher than that of soil culture, increased by about 2%.