Extraction of Effective Compounds in Pine Pollen and In Vitro Assay of their Antitumor Effect
Supercritical CO2 was used to extract effective compounds from pine pollen, MTT colorimetric method was applied to determine anti-cancer effect of effective compounds in pine pollen. Results indicated that extracts obtained at pressure ranges 0~30 MPa and 10~15 MPa had significant inhibition effect on the lung cancer cells 95D of pig (95D) and their IC50 value was 253.22 μg/mL and 252.19 μg/mL, respectively. Extracts attained at pressure ranging 0~10 MPa, 10~15 MPa and 0~30 MPa had significant inhibition effect on the stomach cancer cells SGC-7901 of human (SGC-7901), and their IC50 value was 248.35 μg/mL, 459.56 μg/mL and 301.20 μg/mL, individualy. Supercritical CO2 extract of pine pollen had inhibition effects on the lung cancer cells 95D of pig and the sSubscript texttomach cancer cells SGC-7901 of human, and the corresponding active compounds were different.
Qunjie Xu, Honghua Ge and Junxi Zhang
Z. P. Zhu and N. Li, "Extraction of Effective Compounds in Pine Pollen and In Vitro Assay of their Antitumor Effect", Advanced Materials Research, Vols. 361-363, pp. 731-734, 2012