Extraction of Effective Compounds in Pine Pollen and In Vitro Assay of their Antitumor Effect

Zi Ping Zhu; Na Li

doi:10.4028/www.scientific.net/AMR.361-363.731

Paper Titles

Identiﬁcation and Characteristics of Lactic Acid Bacteria Isolated from Stylosanthes guianensis sw.
p.701

Extraction Mechanism of Total Flavonoids from Red Kidney Bean by Microwave and Light Wave Radiation
p.707

Release of Ferulic Acid from Wheat Bran by Ultrasonic Assisted Alkaline Hydrolysis
p.712

The Research of Ice-Water Phase Change and Heat Transfer in Natural Environment of Northern Winter in China
p.724

Extraction of Effective Compounds in Pine Pollen and In Vitro Assay of their Antitumor Effect
p.731

Experimental Study on the Temperature Variation of a New Tobacco Curing Barn with Double-Way Ventilation
p.735

Optimization of Supercritical CO₂ Extraction of Noni (Morinda citrifolia L) Seed Oil Using Response Surface Methodology
p.743

The Radical Scavenging Activity of Protein Hydrolysates Prepared from Mercenariamercenaria and Ruditapesphilippinarum
p.748

Preliminary Research on Extract Ferulic Acid from Wheat Bran by Ultrasonic-Assisted Alkaline-Alcohol Hydrolysis
p.753

HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 361-363Extraction of Effective Compounds in Pine Pollen...

Extraction of Effective Compounds in Pine Pollen and In Vitro Assay of their Antitumor Effect

Abstract:

Supercritical CO₂ was used to extract effective compounds from pine pollen, MTT colorimetric method was applied to determine anti-cancer effect of effective compounds in pine pollen. Results indicated that extracts obtained at pressure ranges 0~30 MPa and 10~15 MPa had significant inhibition effect on the lung cancer cells 95D of pig (95D) and their IC50 value was 253.22 μg/mL and 252.19 μg/mL, respectively. Extracts attained at pressure ranging 0~10 MPa, 10~15 MPa and 0~30 MPa had significant inhibition effect on the stomach cancer cells SGC-7901 of human (SGC-7901), and their IC50 value was 248.35 μg/mL, 459.56 μg/mL and 301.20 μg/mL, individualy. Supercritical CO₂ extract of pine pollen had inhibition effects on the lung cancer cells 95D of pig and the s_{Subscript text}tomach cancer cells SGC-7901 of human, and the corresponding active compounds were different.