Extraction of Effective Compounds in Pine Pollen and In Vitro Assay of their Antitumor Effect

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Supercritical CO2 was used to extract effective compounds from pine pollen, MTT colorimetric method was applied to determine anti-cancer effect of effective compounds in pine pollen. Results indicated that extracts obtained at pressure ranges 0~30 MPa and 10~15 MPa had significant inhibition effect on the lung cancer cells 95D of pig (95D) and their IC50 value was 253.22 μg/mL and 252.19 μg/mL, respectively. Extracts attained at pressure ranging 0~10 MPa, 10~15 MPa and 0~30 MPa had significant inhibition effect on the stomach cancer cells SGC-7901 of human (SGC-7901), and their IC50 value was 248.35 μg/mL, 459.56 μg/mL and 301.20 μg/mL, individualy. Supercritical CO2 extract of pine pollen had inhibition effects on the lung cancer cells 95D of pig and the sSubscript texttomach cancer cells SGC-7901 of human, and the corresponding active compounds were different.

Info:

Periodical:

Advanced Materials Research (Volumes 361-363)

Edited by:

Qunjie Xu, Honghua Ge and Junxi Zhang

Pages:

731-734

DOI:

10.4028/www.scientific.net/AMR.361-363.731

Citation:

Z. P. Zhu and N. Li, "Extraction of Effective Compounds in Pine Pollen and In Vitro Assay of their Antitumor Effect", Advanced Materials Research, Vols. 361-363, pp. 731-734, 2012

Online since:

October 2011

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$35.00

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