Iron Oxide Nanoparticles Conjugated Monoclonal Antibody for Immunochromatographic Strip Test

Mohamad Nor Noorhashimah; Dyana Zakaria Nor; Azlan Abdul Aziz; Rahmah Noordin; Abdul Razak Khairunisak

doi:10.4028/www.scientific.net/AMR.364.30

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Raman Spectroscopy and Field Emission Scanning Electron Microscopic Studies on Carbon Nanomaterials
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Nanostructured of Vanadium Phosphorus Oxide (Vpo) Catalyst Prepared by Sonochemical Treatment
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Iron Oxide Nanoparticles Conjugated Monoclonal Antibody for Immunochromatographic Strip Test
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HomeAdvanced Materials ResearchAdvanced Materials Research Vol. 364Iron Oxide Nanoparticles Conjugated Monoclonal...

Iron Oxide Nanoparticles Conjugated Monoclonal Antibody for Immunochromatographic Strip Test

Abstract:

In this study, the synthesis of iron oxide nanoparticles (IONPs) and immunochromatographic (ICG) strip test of iron oxide conjugated with monoclonal antibody (IONPs-Ab) have been developed. The IONPs were synthesised using precipitation method and dispersed in water by applying polyethylene glycol coating. Several parameters that affected conjugations of the IONPs-Ab were studied namely iron oxide concentration, antibody volume, stabilizer concentration and the amount of SiPEG coating. From the transmission electron microscopy (TEM) image, the size of IONPs obtained was ~14 nm. Conjugate was tested with the ICG strip test lined with the antigen. The results showed 1.0μl SiPEG coating, 1 M IONPs concentration, 20μl of 1mg/ml antibody volume and 1% bovine serum albumin (BSA) were the optimum. Moreover, the IONPs-Ab was also tested with the Brugian filariasis positive and negative serum samples. The results showed positive result for the patient serum and negative result for the non-patient serum in just 15 minutes.

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Advanced Materials Research (Volume 364)

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30-34

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https://doi.org/10.4028/www.scientific.net/AMR.364.30

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October 2011

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Mohamad Nor Noorhashimah, Dyana Zakaria Nor, Azlan Abdul Aziz, Rahmah Noordin, Abdul Razak Khairunisak

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