Optimization of Overproducing S-Adenosyl-L-Methionine Saccharomyces Cerevisiae S-W55 Mutant Utilizing Unpolished Rice from Aging Paddy by Feeding L-Methionine

Abstract:

The medium and fed-batch fermentation by Saccharomyces cerevisiae S-W55 were optimized. The unpolished rice from aging paddy was used as major nutrient source to reduce the raw material cost of SAM fermentation. The unpolished rice saccharificate (URS) and yeast extract were employed as carbon source and nitrogen source, respectively. The dosages of URS and yeast extract in the medium compositions were optimized by response surface methodology (RSM). As a result, when the fermentation was carried out under the optimal conditions for URS (51.4 g/L) and yeast extract (4.74 g/L), the SAM yield reached 2.61 g/L. Some fed-batch processes by adding L-methionine (MET) were investigated. Adding MET into the fermentation broth at one time at the time of high cell density reaching 80 g/L could get better results with the optimal SAM concentration of 5.3 g/L and biomass yield of 89.1 g/L. By feeding MET into the fermentation broth at a feeding rate of 2 g/h for 5 hours at the time of high cell density reaching 80 g/L the optimal results were reached and the maximal SAM yield and biomass density were 5.82 g/L and 90.2 g/L，respectively.

• Periodical: Advanced Materials Research (Volume 365)
• Pages: 445-453
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.365.445
• Online since: October 2011
• Authors: Wen Jun Wang
• Keywords: Fed-Batch Fermentaion, Fermentation Optimization, Saccharomyces cerevisiae, S-Adenosyl-L-Methionine, Unpolished Rice Saccharificate

References

[1] Das M, Banerjee R, Bal S (2008). Evaluation of physicochemical properties of enzyme treated brown rice (Part B). LWT -Food Sci. Technol. 41, 2092-(2096).

DOI: 10.1016/j.lwt.2007.11.018

[2] Fukushima K, Sogo K, Miura S, Kimura Y (2004). Production of D-lactic acid by bacterial fermentation of rice starch. Macromol. Biosci. 4, 1021-1027.

DOI: 10.1002/mabi.200400080

[3] He JY, Deng JJ, Zheng YH, Gu J (2006). A synergistic effect on the production of S-adenosyl-l-methionine in Pichia pastoris by knocking in of S-adenosyl-l-methionine synthase and knocking out of cystathionine-synthase. J. Biotechnol. 126, 519-527.

DOI: 10.1016/j.jbiotec.2006.05.009

[4] Heerink N, Qu FT, Kuiper M, Shi XP, Tan SH (2007). Policy reforms, rice production and sustainable land use in China: a macro-micro analysis. Agr. Syst. 94, 784-800.

DOI: 10.1016/j.agsy.2006.11.005

[5] John RP, Sukumaran RK, Nampoothiri KM, Pandey A (2007).

[6] Lee CW (2007). Production of D-lactic acid by bacterial fermentation of rice. Fiber Polym. 8: 571-578.

[7] Liang JF, Han BZ, Nout MJR, Hamer RJ (2008). Effects of soaking, germination and fermentation on phytic acid, total and in vitro soluble zinc in brown rice. Food Chem. 110, 821-828.

DOI: 10.1016/j.foodchem.2008.02.064

[8] Lin JP, Tian J, You JF, Jin ZH, Xu ZN, Cen PL (2004). An effective strategy for the co-production of S-adenosyl-l-methionine and glutathione by fed-batch fermentation. Biochem. Engin. J. 21, 19–25.

DOI: 10.1016/j.bej.2004.04.013

[9] Liu H, Lin JP, Wu JP, Cen PL (2002). Production of S-adenosyl-lmethionine using Saccharomyces cerevisiae by bioconversion of L-methionine. Chem. React. Eng. Tech. (In Chinese) 18 (4), 310-314.

[10] Lu ZD, Lu MB, He F, Yu LJ (2009). An economical approach for D-lactics acid production utilizing unpolished rice from aging paddy as major nutrient source. Bioresou. Technol. 100, 2026-(2031).

DOI: 10.1016/j.biortech.2008.10.015

[11] Miller GL (1959). Use of dinitro salicylic acid reagent for determination of reducing sugar. Ann. Chem. 31, 426-428.

[12] Mincheva KP, Balutsov VM (2002). Isolation of a Kluyveromyces lactis Mutant Enriched in S-Adenosyl-L-Methionine and Growing in Whey Medium. Appl. Biochem. Microbiol. 38(4), 335-338.

[13] Shimizu S, Yamada H (1984). Microbial and enzymatic processes for the production of pharmacologically important nucleosides. Trends Biotechnol. 2, 137-141.

DOI: 10.1016/0167-7799(84)90023-4

[14] Shiomi N, Fukuda H, Fukuda Y, Murata K, Kimura A (1990). Production of S-adenosylmethionine by Saccharomyces cerevisiae cells carying a gene for ethionine resistance. Biotechnol. Bioeng. 35, 1120–1124.

DOI: 10.1002/bit.260351107

[15] Wang WJ, Xiao J (2007). Study on Screening S-adenosyl-L-methionine -hyperproducing mutant and Cultivation Conditions by Saccharomyces cerevisiae. J. Wuhan Polytechnic University (In Chinese) 26(4), 17-21.

[16] Zhang JG, Wang XG, Zhang JN, Wei DZ (2008). Oxygen vectors used for S-adenosylmethionine production in recombinant Pichia pastoris with sorbitolas supplemental carbon source. J. Biosci. Bioeng. 105(4), 335-340.

DOI: 10.1263/jbb.105.335