Optimization of Overproducing S-Adenosyl-L-Methionine Saccharomyces Cerevisiae S-W55 Mutant Utilizing Unpolished Rice from Aging Paddy by Feeding L-Methionine

Abstract:

Article Preview

The medium and fed-batch fermentation by Saccharomyces cerevisiae S-W55 were optimized. The unpolished rice from aging paddy was used as major nutrient source to reduce the raw material cost of SAM fermentation. The unpolished rice saccharificate (URS) and yeast extract were employed as carbon source and nitrogen source, respectively. The dosages of URS and yeast extract in the medium compositions were optimized by response surface methodology (RSM). As a result, when the fermentation was carried out under the optimal conditions for URS (51.4 g/L) and yeast extract (4.74 g/L), the SAM yield reached 2.61 g/L. Some fed-batch processes by adding L-methionine (MET) were investigated. Adding MET into the fermentation broth at one time at the time of high cell density reaching 80 g/L could get better results with the optimal SAM concentration of 5.3 g/L and biomass yield of 89.1 g/L. By feeding MET into the fermentation broth at a feeding rate of 2 g/h for 5 hours at the time of high cell density reaching 80 g/L the optimal results were reached and the maximal SAM yield and biomass density were 5.82 g/L and 90.2 g/L,respectively.

Info:

Periodical:

Edited by:

Barry Tan

Pages:

445-453

DOI:

10.4028/www.scientific.net/AMR.365.445

Citation:

W. J. Wang "Optimization of Overproducing S-Adenosyl-L-Methionine Saccharomyces Cerevisiae S-W55 Mutant Utilizing Unpolished Rice from Aging Paddy by Feeding L-Methionine", Advanced Materials Research, Vol. 365, pp. 445-453, 2012

Online since:

October 2011

Authors:

Export:

Price:

$35.00

In order to see related information, you need to Login.

In order to see related information, you need to Login.