Fabrication of Hydroxyapatite and Observation of Nanoparticles Entering into Cells

Abstract:

Unambiguous and thorough knowledge of interactions between cells and nanoparticles was necessary for applications of nanoparticle in living system. In this report we presented systematic studies of fabrication of inorganic nanoparticles and the application of entering into cells. Size distribution, zeta potential and transmission electron microscope (TEM) were performed to characterize the nanoparticles structure and define the mechanism by which nanoparticles are capable of entering into cells. The study revealed nanoparticles can entered cells via penetrating through the lipophilic bilayer, which should be paid attention to for inspirational value insome application researches and will be harmful in other cases.

• Periodical: Advanced Materials Research (Volume 366)
• Pages: 451-455
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.366.451
• Online since: October 2011
• Authors: Dong Yang, En Jie Sun
• Keywords: Cells, Hydroxyapatite (HAP), Penetration

References

[1] L. Chen, J.M. Mccrate, J.C. Lee and H. Li, Nanotechnology, 10 (2011) 105708.

[2] T.H. Chung, S.H. Wu, M. Yao, C.W. Lu, Y.S. Lin, Y. Hung, C.Y. Mou, Y.C. Chen and D.M. Huang, Biomaterials, 19 (2007) 2959.

[3] D.A. Wahl and J.T. Czernuszka, Eur Cell Mater, (2006) 43.

[4] I. Ono, T. Yamashita, H.Y. Jin, Y. Ito, H. Hamada, Y. Akasaka, M. Nakasu, T. Ogawa and K. Jimbow, Biomaterials, 19 (2004) 4709.

DOI: 10.1016/j.biomaterials.2003.11.038

[5] X. Zhu, Y. Wang and J. Ye, BULLETIN OF THE CHINESE CERAMIC SOCIETY, 2 (2003) 77.

[6] G. Li, Y. Zhang, H. Zhang, J. Li and K. Wei, CHINA JOURNAL OF MODERN MEDICINE, 4 (2007) 389.

[7] S. Madhavi, C. Ferraris and T.J. White, J Solid State Chem, 9 (2005) 2838.

[8] K. Kandori, S. Oda, M. Fukusumi and Y. Morisada, Colloids Surf B Biointerfaces, 1 (2009) 140.

[9] P. Zhu, Y. Masuda and K. Koumoto, Biomaterials, 17 (2004) 3915.

[10] M. Motskin, D.M. Wright, K. Muller, N. Kyle, T.G. Gard, A.E. Porter and J.N. Skepper, Biomaterials, 19 (2009) 3307.

DOI: 10.1016/j.biomaterials.2009.02.044

[11] P.N. Kumta, C. Sfeir, D.H. Lee, D. Olton and D. Choi, Acta Biomaterialia, 1 (2005) 65.

[12] Y. Zhai, Y. Gao, F. Liu, Q. Zhang and G. Gao, Mater Lett, 28 (2007) 5056.

[13] S.H. Teng, E.J. Lee, P. Wang and H.E. Kim, Mater Lett, 17-18 (2008) 3055.

[14] A. Jordan, R. Scholz, P. Wust, H. Schirra and Others, J Magn Magn Mater, 1-3 (1999) 185.

[15] S. Prijic and G. Sersa, Radiology and Oncology, 1 (2011) 1.

[16] X. Chao, L. Guo, Y. Zhao, K. Hua, M. Peng, C. Chen and Y. Cui, J Drug Target, 3 (2011) 161.

[17] P.H. Yang, X. Sun, J.F. Chiu, H. Sun and Q.Y. He, Bioconjug Chem, 3 (2005) 494.

[18] H.Q. Mao, K. Roy, V.L. Troung-Le, K.A. Janes, K.Y. Lin, Y. Wang, J.T. August and K.W. Leong, J Control Release, 3 (2001) 399.

[19] M. Lavertu, S. Methot, N. Tran-Khanh and M.D. Buschmann, Biomaterials, 27 (2006) 4815.