Preliminary Fractionation and Derivatives Changes of Myoglobin from Tilapia (O. niloticus♀×O. aureus♂) Dark Muscle with Ammonium Sulfate

Abstract:

Ammonium sulfate fractionation was implicated as one of essential steps to purified Myoglobin (Mb).We adopted SDS-PAGE analysis and absorption spectra scanning to demonstrate the effect of ammonium sulfate on Mb and its derivatives. The results shown that protein with the molecular weight above 37.8kDa were dominated in myoglobin extract of tilapia, which can be precipitated with ammonium sulfate. Mb molecular weight of tilapia is about 15.8 kDa, which can be collected by the treatment of ammonium sulfate with saturation between 60%-70% to remove unwanted protein. The absorption spectra show that Mb derivatives could be transferred to met-myoglobin after preliminary fractionating with ammonium sulfate.

• Periodical: Advanced Materials Research (Volumes 393-395)
• Pages: 890-893
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.393-395.890
• Online since: November 2011
• Authors: Jian Wei Cen, Shu Xian Hao, Lai Hao Li, Xian Qing Yang, Hui Huang, Shi Hong, Ya Wei, Jun Yan He
• Keywords: Ammonium Sulfate, Myoglobin, Preliminary Fractionation, Tilapia

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