Evaluation and Optimization of The Factors Affectiing Enzymatic Hydrolysis of Silk Fibroin

Abstract:

Enzymatic hydrolysis of silk fibroin is studied in detail by both single factor experiments and orthogonal experiments. The results of single factor experiments show that enzyme concentration, temperature and pH value have significant effects on the hydrolysis. With the changes of them, the structures of enzyme molecules will also change, which will cause different hydrolysis rates. According to the results of orthogonal experiments, the degree of influence of these three factors can be sorted in descending order: temperature, concentration and then pH value. The optimized conditions of hydrolysis achieved in this paper are enzyme concentration 0.5g/L, temperature 60°C and pH value 8.60.

• Periodical: Advanced Materials Research (Volumes 399-401)
• Pages: 1536-1539
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.399-401.1536
• Online since: November 2011
• Authors: Lei Zhong, Bing Lu, An Ping Liao
• Keywords: Enzymatic Hydrolysis, Evaluation, Optimization, Silk Fibroin

References

[1] L.X. Deng, L.J. Zhu, H.P. Zhang and S.J. Ming: North Sericulture. Vol. 30 (2009), p.11

[2] C.J. Chen, Z.F. Liu, Q.N. Lou, Y.Y.Gu, X.Q Shi and Y.P. Zhang: North Sericulture. Vol. 29 (2008), p.7

[3] P.Feng: Food Research and Development. Vol. 25 (2004), p.51