A Novel Nonionic, Multi-Surfactant System and Separation Method for the Synthesis of Active Carbonic Anhydrase Nanoparticles

Guo Chao Nie; Di Si; Gwang Seong Kim; Zhong You Shi; Tanvi Siraj Ratani; Yong Eun Koo Lee; Raoul Kopelman

doi:10.4028/www.scientific.net/AMR.399-401.509

Paper Titles

Generic Geometric Model for 3D Woven Interlock Composites
p.478

The Influence of Clay-Like Sepiolite on the Structure and Properties of Polyurethaneurea/Sepiolite Nanocomposites
p.486

The Application Finite Element Analysis with the Temperature Field Simulation of Flame-Retardant Effect on OMMT and APP for PP/PA6 Composts
p.493

An Experimental-Numerical Approach of the Metallic Insert in CFRP/Honeycomb Sandwich Structures under Normal Tensile Load
p.500

A Novel Nonionic, Multi-Surfactant System and Separation Method for the Synthesis of Active Carbonic Anhydrase Nanoparticles
p.509

Low Temperature Combustion Synthesis and Characterization of Pr-CeO₂ Doped with Zr Red Ceramic Pigments
p.514

Influences of Tm and N Doping on Surface Properties and Photoactivities of Anatase-TiO₂ Nanoparticles
p.519

Controlled Synthesis of Water Soluble Silver Nanoparticles and the Molluscicidal Effect
p.527

Fabrication and Characterizes of TiO₂ Nanomaterials Templated by Lyotropic Liquid Crystal
p.532

HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 399-401A Novel Nonionic, Multi-Surfactant System and...

A Novel Nonionic, Multi-Surfactant System and Separation Method for the Synthesis of Active Carbonic Anhydrase Nanoparticles

Abstract:

This paper describes a new synthesis and separation method of nanoparticles (NPs) using a non-toxic, non-ionic surfactant systems. The purification steps did not use ethanol or acetone. Results indicate that the wild type bovine Carbonic Anhydrase (CA) activity was enhanced almost 4 times more than the CA encapsulated NPs fabricated by the traditional method. The NPs have are more hydrophilic and also have a higher zeta potential. The well dispersed CA PAA NPs with of 10-30 nm in diameter were obtained. This work also demonstrates a universal method for immobilizing fragile biomacromolecules in NP carriers for biomedical applications.

You might also be interested in these eBooks

New Materials, Applications and Processes

View Preview

Info:

Periodical:

Advanced Materials Research (Volumes 399-401)

Pages:

509-513

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.399-401.509

Citation:

Cite this paper

Online since:

November 2011

Authors:

Guo Chao Nie, Di Si, Gwang Seong Kim, Zhong You Shi, Tanvi Siraj Ratani, Yong Eun Koo Lee, Raoul Kopelman

Keywords:

Active Enzyme, Carbonic Anhydrase, Encapsulation, Fragile Biomacromolecules, Nanoparticle, Nonionic Surfactants

Export:

RIS, BibTeX

Price:

Permissions CCC:

Request Permissions

Permissions PLS:

Request Permissions

Сopyright:

Citation:

References

[1] Charles L. Wittenberger, Alfred J. Beaman, Linda N. Lee: J. Bacteriol. Vol. 1(1978), P. 231-239.

Google Scholar

[2] Hung, B. R., L. Lara, M. A. Patron, N. N. Ugarova, W. Bechstedt, and S. Clappes: Acta Biotechnol.Vol. 8(1988), P. 461–464.

DOI: 10.1515/9783112581780-015

Google Scholar

[3] Kato K, Walde P, Mitsui H, Higashi N: Biotechnol Bioeng. Vol. 84(2003), P. 415-423.

Google Scholar

[4] W. G. ZHANG and X. Y. GE: Food Technol. Biotechnol.Vol. 44(2006), P. 59–64.

Google Scholar

[5] Koziara Joanna M, Lockman Paul R, Allen David D: J. Controlled Rel. Vol.99( 2004),P. 259-269.

Google Scholar

[6] Jia Liu, Jingang Shi, Jian Li, Xingzhong Yuan: Computer Distributed Control and Intelligent Environmental Monitoring (CDCIEM), 2011 International Conference, P.1207 – 1210.

Google Scholar

[7] Khoo, M. L. and Ibrahim, C. O, Malaysian Journal of Microbiology, Vol. 15(2009),P.1-5.

Google Scholar

[8] Hironobu Kunieda and Kózó Shinoda: J. Colloid and Interface Sci.,Vol. 107(1985),P. 107-121.

Google Scholar

[9] Fuminori Harusawa , Hideo Nakajima , Muneo Tanaka: J. Soc. Cosmet. Chem. Vol.33 (1982), P.115-129.

Google Scholar

[10] Griffin, William C: J. Soc. Cosmet. Chem.Vol. 1(1949), P. 311-326.

Google Scholar

[11] Goh SL, Murthy N, Xu M, Frechet JMJ: Bioconj. Chem. Vol.15 (2004), P. 467-474.

Google Scholar

[12] Ponisseril Somasundaran, Soma Chakraborty, U. S. Patent, 20080260851. (2008)

Google Scholar

[13] G. Ramachandra, Nandanan Erathodiyil, U. S. Patent, 20050196343. (2005)

Google Scholar

[14] A. Shome, S. Roy, and P. K. Das, Langmuir,Vol.23(2007),P. 4130-4136.

Google Scholar

[15] Y. Liang, X. Yuan, G. Zeng, H. Zhong, H. Li, W. Wang: Science China Chemistry, Vol. 54(2011), P.715-723.

Google Scholar