Extraction Optimization of Antibacterial Substances from Amomum tsao-ko

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Abstract:

The aim of this work was to optimize extraction procedure of antibacterial compounds from amomum tsao-ko and verify antibacterial ability of yields. Two methods were carried out and compared. One is ethanol reflux extraction, and the other is ultrasonic-assisted ethanol extraction which selected due to better antibacterial efficiency of the extract obtained. In order to improve extraction rate and antibacterial activity which both varied with ultrasonic-assisted extraction conditions, Box-Behnken design was employed. Studies showed that the optimum conditions for extraction antibacterial substances from amomum tsao-ko were determined to be: ethanol density 100%, soaking time 4h, extraction time 120min.

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Advanced Materials Research (Volumes 430-432)

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1036-1039

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January 2012

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© 2012 Trans Tech Publications Ltd. All Rights Reserved

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