Chitosan Metal Complex: An Artificial Metalloprotease Hydrolyzing Trypsin Inhibitor from Soybean

Peng Liang; Dong Feng Wang; Xing Yu Wang; Mei Ding; Li Zhang; Ying Xu

doi:10.4028/www.scientific.net/AMR.430-432.414

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Chitosan Metal Complex: An Artificial Metalloprotease Hydrolyzing Trypsin Inhibitor from Soybean
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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 430-432Chitosan Metal Complex: An Artificial...

Chitosan Metal Complex: An Artificial Metalloprotease Hydrolyzing Trypsin Inhibitor from Soybean

Abstract:

Most popular agents for protein cleavage are proteolytic enzymes, but they require more rigorous hydrolytic conditions, and it is very difficulty to separate them from the productions. In this paper, an new artificial metalloprotease, Cu(II) complex of cyclen (Cu(II)Cyc) using chitosan as supporter, was designed and synthesized successfully. The hydrolytic efficient under different conditions was measured and confirmed with HPLC, SDS-PAGE, further more. Some reaction conditions, such as pH, temperature, were researched, and the results show that the optimum reaction time was 48 h, temperature was 60 °C respectively, The observed rate constants for trypsase inhibitor cleavage was 1.006×10-2 h-1 under above conditions, and the observed rate constants was 2.120×10-2 h-1 when the pH was 9.0. This paper suggested that the chitosan metal complexes could efficiently accelerate the hydrolysis reaction.

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Advanced Materials Research (Volumes 430-432)

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414-418

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https://doi.org/10.4028/www.scientific.net/AMR.430-432.414

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Online since:

January 2012

Authors:

Peng Liang, Dong Feng Wang, Xing Yu Wang, Mei Ding, Li Zhang, Ying Xu

Keywords:

Hydrolysis Hydration, Metal Complex, Metalloprotease, Trypsase Inhibitor

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