Kinetic Effect of Cationic Comb-Type Copolymers on DNA Hybridization

Atsushi Maruyama; Long Liang Wu; Naohiko Shimada; Arihiro Kano

doi:10.4028/www.scientific.net/AMR.47-50.1355

Paper Titles

An Evaluation of CaF₂ Doping Tricalcium Silicate as Dental Restorative Materials
p.1339

Construction of Nonbiofouling Biofunctional Glass Surface by Self-Assembled Monolayer and Graft Hydrophilic Polymer
p.1343

Poly(L-Lactide)/Multiwalled Carbon Nanotube Composites: Interaction with Osteoblast-Like Cells In Vitro
p.1347

In Vitro Bioactivity of Potassium Titanate Whiskers
p.1351

Kinetic Effect of Cationic Comb-Type Copolymers on DNA Hybridization
p.1355

Bio-Fabrication of Patterned Cellulose Nano-Fibers
p.1359

Electrospinning Nanocomposite Nanofibers of Hydroxyapatite/Chitosan
p.1363

Plasma Polymerized Thin-Films for Biosensors
p.1367

The Design of Novel Scaffolds by Integrating Microbial Cellulose onto Plasma Treated Polypropylene
p.1371

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Kinetic Effect of Cationic Comb-Type Copolymers on DNA Hybridization

Abstract:

We have been interested in the soluble interpolyelectrolyte complexes (IPEC) between DNA and the cationic comb-type copolymers, poly(L-lysine)-graft-dextran (PLL-g-Dex), consisting of a polylysine backbone and abundant hydrophilic graft chains of dextran as a model of nucleic acid-binding proteins. In this paper, we described the kinetic effect of the copolymers on the DNA hybridization. We found that the cationic copolymer at nano molar concentrations in cationic groups accelerated DNA duplex formation by two orders under physiologically relevant conditions.