The Separation Identification and Culture Optimization of Hydrogen Production X9 Bacteria

Hong Xu Bao; Wei Wei Cai; Xi Ping Ma; You Tao Song; Man Li Shen; Zhong Lin Chen; Li Dan Li; Nan Qi Ren

doi:10.4028/www.scientific.net/AMR.512-515.1446

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 512-515The Separation Identification and Culture...

The Separation Identification and Culture Optimization of Hydrogen Production X9 Bacteria

Abstract:

A high efficiency simultaneous cellulose degradation and hydrogen production strain X9 was obtained from the screening of the 125 strains of fermentative hydrogen production bacteria which were isolated from a continuous stirred-tank hydrogen production bioreactor (CSTR) with LM-1 and microcrystalline cellulose (MCC) medium and improved Hungered technique in this study. X9 was confirmed a new category by analyzing the results of biochemical and physiological test, shape character, 16S rDNA sequencing and phylogenetic, and then analysis characteristics of growth, culture parameters, the effects of components of medium on growth and ability of hydrogen production, the optimum technological parameters have been determined from orthogonal experiment and single factor test. Finally, X9 achieved maximum specific hydrogen yield of 4.9mmol/g with MCC under the optimal conditions.

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Periodical:

Advanced Materials Research (Volumes 512-515)

Pages:

1446-1449

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.512-515.1446

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Online since:

May 2012

Authors:

Hong Xu Bao, Wei Wei Cai, Xi Ping Ma, You Tao Song, Man Li Shen, Zhong Lin Chen, Li Dan Li, Nan Qi Ren

Keywords:

Biohydrogen Production, Cellulose Degradation, Hydrogen-Producing Bacteria

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