Selective Breeding and Application of Hypercellulolytic Mutant Strains

Jie Nan Chen; Lin Zhang; Ling Ling Qin; Cui Can Wang; Gang He; Peng Zhan

doi:10.4028/www.scientific.net/AMR.518-523.5417

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Selective Breeding and Application of Hypercellulolytic Mutant Strains
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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 518-523Selective Breeding and Application of...

Selective Breeding and Application of Hypercellulolytic Mutant Strains

Abstract:

For obtaining hypercellulolytic strains, Trichoderma reesei Rut C30 was treated by UV irradiation. After twice selection and 7 subcultures conduction, A5, A9, A13 and A22 four mutant strains were abtained, and their filter paper activity (FPA) increased 31.01%, 29.83%, 55.35%, 32.27% respectively than parent strain. Random amplified polymorphic DNA analysis (RAPD) demonstrated there was existed genetic diversity between the mutants. The maximum filter paper activity (FPA) of A13 achieved 6.09 U/ml by central composite design (CCD). For applying into poplar hydrolysis, the mixed cellulase of A13 and Eupenicillium javanicum ZN-205 resulted in superior production of reducing sugar than its single cellulase counterparts. The maximum reducing sugar production was 25.75g/L in 5% substrate, and the corresponding saccharification rate was 78.18%.

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Advanced Materials Research (Volumes 518-523)

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5417-5422

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https://doi.org/10.4028/www.scientific.net/AMR.518-523.5417

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Online since:

May 2012

Authors:

Jie Nan Chen, Lin Zhang, Ling Ling Qin, Cui Can Wang, Gang He, Peng Zhan

Keywords:

Cellulase, Optimization, RAPD, Trichoderma reesei, UV Mutagesis

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References

[1] N.J. Nagle, R.T. Elander M.M. Newman B.T. Rohrback, and R.W. Torgetm: Biotechnol. Progr., vol. 18 (2002), pp.734-738.

Google Scholar

[2] F.E. Alexander, P.J. Richard, T.T. Brian, J.D. Aandrew, H.O. Michael and K.M. Daniel: Science, vol. 311 (2006), pp.506-508.

Google Scholar

[3] F.W. Bai W.A. Anderson and M.Moo-Young: Biotechnol. Adv., vol.26 (2008), pp.89-105.

Google Scholar

[4] J.N. Chen, L. Zhang, P. Zhan, Y.Q. Wang, B.L. Ai, and C.C. Wang: Advances in Biomedical Engineering, edtied by J. Hu Information Engineering Research Institute Publishing, Delaware, USA. (2011), pp.295-298.

Google Scholar

[5] Miettinen-Oinonen A and Suominen P. : Appl Environ Microbiol, vol.68 (2002), p.3956–3964.

Google Scholar

[6] X.H. Li, H.J. Yang, B. Roy, E.Y. Park, L.J. Jiang, D. Wang, Y.G. Miao: Microbiolgical Reseach, vol.165 (2010), pp.190-198.

Google Scholar

[7] M.G. Adsul, K.B. Bastwede, A.J. Varma, D.V. Gokhal: Bioresource Technology, vol.98 (2007), pp.1467-1473.

Google Scholar

[8] D. Sternberg, P. Vijayakumar, and E. T. Reese: Can. J. Microbiol., vol. 23 (1977), p.139–147.

Google Scholar

[9] T. K. Ghose: Pure Appl. Chem., vol. 59 (1987), p.257–268.

Google Scholar

[10] M. Ferreira, D.Grattapaglia: Introduçao uso de marcadores moleculares em análise genética. 1st ed. Brasília: EMBRAPA-CENARGEN; 1995.

Google Scholar

[11] Rohlf FJ. NTSYS-pe numerical taxonomy and multivariate analysis system version 2.2, Exeter software, Applied biostatics. New York; 2004.

Google Scholar

[12] Sneath PHA, Sokal RR. Numerical taxonomy. The principle and practice of numerical classification. San Francisco: W.H. Freemon and Company; 1973.

Google Scholar

[13] G.L. Miller: Anal. Chem. Vol. 31 (1959), pp.426-438.

Google Scholar

[14] H.B. Liu, Y.Q. Wang, J.N. Chen, W.T. Zhang, H. Xiao: Journal of Biology. Vol. 25 (2008), pp.16-21 (In Chinese).

Google Scholar