Degradation and Ecological Functions of RubiscoLSU during Severe Drought Stress Leaves of Camptotheca acuminata

Abstract:

Cultivated C. acuminata was distributed widely in arid area belonging to middle part of China where plant medicinal material industrials were highly developed due to limited wild resources. It was showed that drought situation could result in rapid decrease of leaf RWC lower than 50% after 6 hours of treatment and exerted adverse effects on growth and development of C. acuminata seedlings. In this paper, proteomics was used to investigate protein changes and their ecological adaptation to desiccation with the treatment of 15% PEG8000 solute to one-year C. acuminata seedlings. 2-DE was adopted to reflect total protein changes after 0 and 30 min, 3h and 5h of treatment and the different expressing blots was identified using PMF attempting to demonstrate damage mechanism of desiccation to plants and ecologically adapting. The results showed that the degradation of Rubisco large subunit (Rubisco LSU) occurred and HSP70 was implicated in the modulation process within 5 h treatment.

• Periodical: Advanced Materials Research (Volumes 518-523)
• Pages: 5429-5435
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.518-523.5429
• Online since: May 2012
• Authors: Jing Hua Yu, Shu Sheng Yuan, Zhong Hua Tang, De Wen Li, Yuan Gang Zu
• Keywords: Camptotheca Acuminata, Ecological Adaptation, LSU of Rubisco

References

[1] R. Zhang, Y. Li ,Q. Cai, T. Liu, H. Sun, B. Chambless: Cancer Chemoth. Pharm. Vol.41 (1998), pp.257-267

[2] E. A. Bray: Plant Physiol. 1993, Vol.103 (1999), pp.1035-1040

[3] J. Flexas, M. Ribas-Carbó, J. Bota, J. Galmés, M. Henkle, S. Martínez-Cañellas and H. Medrano: New Phytol. Vol.172 (2006), pp.73-82

DOI: 10.1111/j.1469-8137.2006.01794.x

[4] W. Wang, M. Scali, R. Vignani, A. Spadafora, S. Mazzuca and M. Cresti: Electrophoresis Vol.24 (2003), pp.2369-2375

DOI: 10.1002/elps.200305500

[5] B. P. Mooney and J. J. Thelen: Phytochemistry, Vol.65 (2004), pp.1733-1744

[6] L. C. Packman: Meth. Mol. Cell. Biol. Vol.4 (1993), p.189–198

[7] Q. Y. Xiang, D. E. Soltis, D. R. Morgan and P. S. Soltis: Ann. Mo. Bot. Gard. Vol.80 (1993), pp.723-734

[8] M. A. J. Parry, P. J. Andralojc, S. Khan, P. J. Lea and A. J. Keys: Ann. Bot-London Vol.89 (2002,) p.833–839

[9] S. Komatsu, H. Karibe, T. Hamada and R. Rakwal: Theor. Appl . Genet. Vol.98 (1999), pp.1304-1310

DOI: 10.1007/s001220051196

[10] H. Ishida, S. Shimizu, A. Makino and T. Mae: Planta Vol.204 (1998), pp.305-309

[11] C.B. Wiese and E.J. Pell: Plant Cell Environ. Vol.20 (1997), p.1283–1291

[12] K. Demirevska-Kepova, L. Simova-Stoilova,Z. Stoyanova, R. Hölzer and U. Feller: Environ Exp Bot. Vol.52 (2004), pp.253-266

DOI: 10.1016/j.envexpbot.2004.02.004

[13] N.Kokubun, H. Ishida, A. Makino and T. Mae: Plant Cell Physiol. Vol.43 (2002), pp.1390-1395

[14] M. Desimone, A. Henke and E. Wagner: Plant Physiol.Vol.113 (1996), pp.789-796

[15] G. Noctor, S. Verjovic-jovanovic, S. Driscoll, L. Novitskaya and C. H. Foyer: Ann:Bot-London Vol.89 (2002), pp.841-850