Study on Extraction of Total Flavonoids from Licorice with Ultrasonic Assistance and Antioxidant Activity

Abstract:

To optimize the extraction process of total flavonoids from RADIX RHIZOMA GLYCYRRHIZAE and Study on the Antioxidant Activity. Total flavonoids were extracted from RADIX RHIZOMA GLYCYRRHIZAE with ethanol by ultrasonic-assisted method, whose extraction process was optimized by orthogonal test design.The scavenging effect of pumpkin polysaccharide on OH and O2-were studied by salicylic acid and pyrogallic acid method. The optimum extraction process was as follows: ethanol concentration of 75%, solid-liquid ratio of 1:25,ultrasonic power of 350 W and ultrasonic duration of 25 min.Under the above conditions, the extraction rate of total flavonoids reached as high as 1.943%. The influencing order of each factor on extraction rate of polysaccharides was determined to be: ethanol concentration＞solid-liquid ratio＞ultrasonic power＞ultrasonic duration. When the solution concentration is 1.1296mg/ml, the scavenging rate of crude flavonoids on superoxide radical is 50%.Suggesting that it would be an effctive natural free radical scavenger. The study could provide a scientific basis for the further exploitation and utilization of RADIX RHIZOMA GLYCYRRHIZAE

• Periodical: Advanced Materials Research (Volumes 550-553)
• Pages: 1629-1632
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.550-553.1629
• Online since: July 2012
• Authors: Yu Hong Zou, Qiu Xia Han, Lin Guo
• Keywords: Free Radical, Optimization, RADIX RHIZOMA GLYCYRRHIZAE, Total Flavonoid, Ultrasonic

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