Establishment of Multiple-PCR for Diagnosing Three Food-Borne Pathogenic Bacteria in Aquatic Products

Hui Li Xia; Hui Zheng; Jin Jun Zhang

doi:10.4028/www.scientific.net/AMR.554-556.1029

Paper Titles

Determination of Nitrofurans Residue in Fishery Products Using High Performance Liquid Chromatography
p.1013

Changes of Corn Flour by Different Treatments
p.1017

Comparison and Analysis of Acid-Stable and Acid-Unstable α-Amylases from Aspergillus niger with Computer
p.1021

Analysis of Nutrition Content in Longan Seeds
p.1025

Establishment of Multiple-PCR for Diagnosing Three Food-Borne Pathogenic Bacteria in Aquatic Products
p.1029

Physical Properties of Resistant Mungbean Starch Prepared by Enzyme and Acid Treatment
p.1033

Physicochemical Characteristics of Pineapple (Ananas mill.) Peel Cellulose Prepared by Different Methods
p.1038

Characteristics and Antioxidant Activity of Maillard Reaction Products from Porcine Plasma Protein Hydrolysate-Galactose Model System
p.1042

Effects on the Transparency of Corn Starch Dumpling Wrappers
p.1049

HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 554-556Establishment of Multiple-PCR for Diagnosing Three...

Establishment of Multiple-PCR for Diagnosing Three Food-Borne Pathogenic Bacteria in Aquatic Products

Abstract:

A rapid multiplex-PCR method was established in order to detect three foodborne pathogenic bacteria including Salmonella typli，Vibrio parahaemolyticus and Listeria monocytogenes in aquatic products. Three pairs of oligonucleotide primers were designed for multiplex-PCR amplification according to gene coding invasion protein A of Salmonella typli，gene coding immune response of Vibrio parahaemolyticus and regulation histone gene of Listeria monocytogenes. The amplified fragment sizes of these three bacteria were 213 bp, 369 bp and 517bp, respectively．The specificity of the multiplex- PCR was high and the minimum detection limit reached 103 CFU/mL. The multiplex-PCR method was used to analyze three aquatic product samples compared with national standard methods, the coincidence rate of two methods reached 100%. The method developed in this study had high sensitivity and specificity, which could be applied for the rapid detection and molecular epidemiology survey of food-borne pathogenic bacteria in aquatic products．