Determination of Nitrofurans Residue in Fishery Products Using High Performance Liquid Chromatography

Abstract:

Nitrofurans residue in fishery products were dangerous for human health and still remain a big challenge in China. However, there are a few available methods to detect nitrofurans residue in fishery products. This study aimed at developing a high performance liquid chromatography (HPLC) method to detect four nitrofurans (furaltadone, nitrofurazone, nitrofurantoin and furazolidone) residue in fishery products sensitively . Firstly the nitrofurans were extracted from samples with ethyl acetate and then concentrated with rotary evaporator. After mobile phase dissolved and normal hexane defatted, the analytes were quantified with an external standard method by HPLC. The results showed that the linear range was 0.025~1.000μg/mL with correlation coefficients all more than 0.998 and the quantification limits of furaltadone, nitrofurazone, nitrofurantoin, furazolidone, were 11.0, 6.00, 13.0 and 7.00μg/kg respectively. The method was used to detect the nitrofurans in fishery products prepared with recovery rates from 72.2% to 107.4% and relative standard deviation (RSD) varied from 6.2% to 10.4%. Therefore, this method can be used for monitoring nitrofurans in commercial fishery products, improving the safety of commercial fishery products in China.

• Periodical: Advanced Materials Research (Volumes 554-556)
• Pages: 1013-1016
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.554-556.1013
• Online since: July 2012
• Authors: Xuan Yun Huang, Dong Mei Huang, Yong Fu Shi, Bing Feng, Bing Li, Hui Juan Yu, Jie Xu, Yuan Wang, You Qiong Cai
• Keywords: Fishery Products, Food Safety, Furaltadone, Furazolidone, High Performance Liquid Chromatography (HPLC), Nitrofurantoin, Nitrofurazone

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