The Open Culture of Nostoc flagelliforme with a 25 L Open Pond

Abstract:

The open culture is a simple, low-cost way for microalage cultivation, and the great advantage of which is that it can be easily used for mass production. This culture system is primarily used for the alga that can survive under bad or extreme conditions. N. flagelliforme is a kind autotrophic alga having the property of alkali-resistance, which makes open culture of N. flagelliforme become possible. In this study, the cultivation of N.flagelliforme with an open, 60×40×30cm rectangular glass reactor was conducted. Firstly, the open culture of N.flagelliforme was carried on without new nutrient elements supplemented into pond intermittently. During the whole process of cultivation, the pH maintained at 8-9 in order to avoid the contamination of other green alga. At the end of the cultivation, the cell density reached 0.802 g•L^-1 and the exopolysaccharide concentration was 97mg•L^-1. Secondly, the fed-batch cultivation was employed to increase biomass and exopolysaccharide production. At the end of the fed-batch cultivation, the biomass and exopolysaccharide of N.flagelliforme increased distinctly to 1.16 g•L^-1 and 124 mg•L^-1, respectively.

• Periodical: Advanced Materials Research (Volumes 554-556)
• Pages: 1009-1012
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.554-556.1009
• Online since: July 2012
• Authors: Ning Tan, Shi Ru Jia, Pei Pei Han, Wei Guo, Yu Jie Dai
• Keywords: Biomass, Exopolysaccharide, Nostoc Flagelliforme, Open Culture

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