Comparison and Analysis of Acid-Stable and Acid-Unstable α-Amylases from Aspergillus niger with Computer

Yong Le Liu; Fa Xiang Wang; Jian Yu; Xiang Hong Li; Jian Hui Wang

doi:10.4028/www.scientific.net/AMR.554-556.1021

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 554-556Comparison and Analysis of Acid-Stable and...

Comparison and Analysis of Acid-Stable and Acid-Unstable α-Amylases from Aspergillus niger with Computer

Abstract:

Aspergillus niger can produce an acid-stable α-amylase as well as an acid-unstable one, the relation between the structural differences and their acid-resistant capability is of great interest. In this paper, the primary parameters, secondary and tertiary structures of these two types of α-amylases was analyzed and compared, the results shows that they has 67% sequence identity and high homology in secondary and tertiary structure. The acid-stable α-amylase has lower isoelectric point and higher molar percent of acidic amino acid than the acid-unstable one. The four major structural differences of these two types of enzymes is located at residues 132-138, 206-212, 329-335 and 378-382 and formed vary fold pattern.

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Advanced Materials Research (Volumes 554-556)

Pages:

1021-1024

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.554-556.1021

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Online since:

July 2012

Authors:

Yong Le Liu, Fa Xiang Wang, Jian Yu, Xiang Hong Li, Jian Hui Wang

Keywords:

Acid-Stable, Acid-Unstable, Alpha-Amylase, Aspergillus niger, Tertiary Structure

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