Ultra High-Pressure and Ion Implantation Combined Mutagenesis to Improve the Production of β-Carotene from Red Yeast

Abstract:

The Ultra high pressure (UHP) and ion implantation were used to improve the yield of β-carotene from Rhodotorula glutinis NR-98. A mutant G-39 strain was achieved by using ultra high pressure to treat NR-98 strain at a pressure of 300 MPa. The yield of β-carotene increased from the original concentration of 6.03 mg/L to 9.64 mg/L, the increase percentage was 59.87%. Passage experiment indicated it has a good genetic stability. Then, low energy nitrogen ion of 10 Kev was injected to treat the mutant G-39 strain. The other ion mutant GL-5 strain was obtained after screening. The yield of β-carotene was increased from 9.64 mg/L to 17.36 mg/L with an increase percentage of 80.08%. GL-5 strain also has a good genetic stability. The study showed that good mutagenesis effects could be obtained by using combined UHP and ion implantation methods to treat with Rhodotorula glutinis NR-98. The yield of β-carotene was increased from 6.03 mg/L to 17.36 mg/L with a total increase percentage of 188%. The result has a practical application prospect.

• Periodical: Advanced Materials Research (Volumes 554-556)
• Pages: 1165-1169
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.554-556.1165
• Online since: July 2012
• Authors: Sui Lou Wang, Wei Liu, Hai Xiang Wang, Chun Hui Lv
• Keywords: Ion Implantation, Red Yeast, Ultra High Pressure, Yield, β-Carotene

References

[1] Suilou Wang and Haixiang Wang: Food Science, 32 (2011),277-280.

[2] Suilou Wang, Xiaozong Wu, Lihua Hao and Junshe Sun: Acta Microbiologica Sinica, 45 (2005), 970-973.

[3] D.H. Bartlett: Biochemical et Biophysical Acta, 1995 (2002), 367-381.

[4] R.A. Van and F.C. Neidhardt: Proc.Natl.Acad.Sci. 87 (1990), 5589-5593.

[5] E. Chi and D.H. Bartlet: Bacteriol., 175 (1993), 7533-7540.

[6] D.H. Bartlett, C. Kato and K. Horikoshi: Res. Microbiol., 146 (1995) 697-706.

[7] F. Abe and K. Horikoshi: Mol.Cell.Biol., 20 (2000), 8098-8102.

[8] K.J.A. Hauben, D.H. Bartlett and C.C.F. Soontjens: Appl. Environ. Microbiol, 63 (1997), 945-950.

[9] Xiang Gao, Jiong Li and Kangcheng Ruan: Acta Biochimica et Biophysica Sinica, 33 (2001), 77-81.

[10] Gejian Zhu, in: Industrial Microbe Breeding Science, Chemical Industry Press (2006).

[11] Huiyun Feng and Zengliang Yu: Materials Science and Engineering, 54 (2006), 49-120.

[12] W. Barszczewski and M. Robak: Food Microbiol, 21 (2004), 227-231.

[13] Haibo Jiang, Ji Wa ng, Jianmin Yao and Zengliang Yu: Industrial Microbiology, 30 (2000), 1-3.