Characterization and Suppressive Effect of Astragalus Polysaccharide in Glomerulonephritis Rats

Shi Gang Li; Yong Qi Zhang

doi:10.4028/www.scientific.net/AMR.554-556.1713

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 554-556Characterization and Suppressive Effect of...

Characterization and Suppressive Effect of Astragalus Polysaccharide in Glomerulonephritis Rats

Abstract:

A water-soluble polysaccharide named as APS was isolated from the roots of Astragalus membranaceus by hot water extraction, anion-exchange and gel-permeation chromatography and tested for its renal protective effect. Its structural characteristics were investigated by FTIR, AMLC, NMR spectroscopy, GLC–MS, methylation analysis, periodate oxidation and Smith degradation. Based on the data obtained, APS was found to be a α - (1-4)-D -glucan, with a single α- D-glucose at the C-6 position every nine residue, on average, along the main chain. The glucan has a weight-average molecular weight of about 3.6×104 Da. 24h Urine protein quantification and morphological observation, NF-κB protein expression and IL-2, IL-6 and TNF- α concentrations were significantly decreased by APS treatment, clearly suggesting that APS is effective in protecting against glomerulonephritis induced by C-BSA through the inhibition of NF- κB mediated-cytokine pathway.

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Periodical:

Advanced Materials Research (Volumes 554-556)

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1713-1716

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.554-556.1713

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Online since:

July 2012

Authors:

Shi Gang Li, Yong Qi Zhang

Keywords:

Astragalus membranaceus, D-Glucan, Renal Protection, Structure

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