Advanced Materials Research Vols. 554-556

Abstract: Solvent extraction experiment of Sc was investigated.The extraction system consisted of 25% TBP+60% sulfonated kerosene +15% reforming agents. The results show that the extraction rates of Sc can reach up to 95%.The removing of Fe，Mn and Ti can be reached 99.1%, 99.4% and 99.5% respectively.The anti-extraction rates from organic phase with NaOH reach up to 99.7 % with effective separation from Fe，Mn and Ti.

Abstract: A high-performance liquid chromatographic method (HPLC) with evaporative light scattering detection (ELSD) has been developed for fingerprint analysis of Flos Lonicerae. The samples were separated with an Agilent C18 column using acetonitrile (A) and 0.1% formic acid solution under gradient conditions (0→10 min：11.5%→15% A, 10→12 min：15% →22% A, 12→18min：22%→33% A; 18→30min: 33%→45% A) as the mobile phase at a flow rate of 0.8ml min−1 within 30 min. The ELSD conditions were optimized at nebulizer-gas flow rate 2.7 L min−1 and drift tube temperature 105°C. Precision experiments showed relative standard deviation (R.S.D.) of peak area and retention time were better than 2.5%, inter-day and intra-day variabilities showed that R.S.D. was ranged from 0.66% to 4.17%. Accuracy validation showed that average recovery was between 95.33% and 104.10%. The method was validated to achieve the satisfactory precision and recovery. Relative retention time and relative peak area were used to identify the common peaks for fingerprint analysis. There are nine common peaks in the fingerprint. The quality of sixteen batches of Flos Lonicerae samples was evaluated by hierarchical clustering analysis, which classfied all bacthes into two clusters. Furthermore, the contents of important medicinal compounds (chlorogenic acid, macranthoidin B and dipsacoside B) in different batches of Flos Lonicerae samples were determined simultaneously using the developed HPLC-ELSD method. The developed analytical procedure was proved to be a reliable and rapid method for the quality control of Flos Lonicerae.

Abstract: In this research the atomic absorption spectroscopy method (the correlation coefficient was 0.9995) was employed for determination of the content of heavy metal-manganese in panax pseudo-ginseng var. notoginseng, which was chosen as the sample. The optimum conditions of two pretreatments were researched independently. In the dry ashing, the optimized temperature and time are 600°C and 4~6 hours respectively, and the manganese content determined is 53.16 ug/g and the recovery rate of manganese added in sample is 98.64%. In the wet digestion, The HNO3-H2O2 digestion system is the best one, and the content was 52.95ug/g and the recovery rate is 97.30%. So the two pretreatment methods of the sample are accurate enough on manganese content determination. But the dry ashing is better than the wet digestion relatively.

Abstract: The highly overlapped chromatograms and spectra of salicylic acid (SA), gentisic acid (GA) and uncalibrated interferent, pyrocatechuic acid (PA) were resolved using HPLC-DAD combined with a novel second-order calibration method. Their elution time was set from 0.6628 min to 0.9495 min and the detection wavelength was chosen from 268 nm to 332 nm. Both SA and GA were determined simultaneously in aqueous solution with their recoveries were (101.0 ± 7.1) % and (105.8 ± 5.6) %, respectively. The satisfying results demonstrate this method can be easily performed and applied to solving second-order calibration problems quickly and accurately.

Abstract: Trihalomethanes (THMs) and haloacetic acids (HAAs) formation character of two samples collected from a reclaimed water treatment plant (RWTP) in North China was investigated, and the chlorination was found brought a high THMs (0.30~0.36μmol/mmol-DOC) and HAAs (2.38~3.07μmol/mmol-DOC) yields in the samples. In order to control THMs and HAAs formation during reclaimed water chlorination, the impact of ammonia addition (to NH3-N=8.3, 17, 24, 30mg/L), pH adjusting (to pH=4.4, 5.04, 7.93, 8.7) and ozone oxidation (with 5.1, 9.3, 13.6, 17.4mg-O₃/L) before sample chlorination were tested respectively and compared with each other. Finally, ammonia addition (to NH₃-N=17mg/L) was proven with greatest efficacy on DBP formation and bromide incorporation control (TTHM, THAA, THM bromide incorporation and HAA bromide incorporation were reduced by 15.41%, 28.21%, 26.88% and 13.63% respectively).

Abstract: Removal of arsenic ion from fish condiment using lignin as adsorbent was investigated. The effects of pH of aquatic condiment solution, temperature, adsorption time, solid to liquid ratio, salt concentration and amino acid concentration on the removal ratio of arsenic ion from aquatic condiment were also studied. Removal ratio of arsenic ion reached 98.65% with pH 2, temperature 40 °C, adsorption time 25 min, solid to liquid ratio 1:70. It was confirmed that lignin can be used to remove arsenic ion from aquatic condiment.

Abstract: Hypocrellins, natural photosensitizers including hypocrellin A (HA) and hypocrellin B (HB), have been used as a traditional Chinese herbal medicine to cure various skin diseases. Hypocrellins have excellent antiviral activity, which can inhibit the growth of human immunodeficiency virus. They also exhibit significant light-induced antitumor property. In this article, the progress on of free radicals research and conformational research Hypocrollins is introduced.

Abstract: Organically modified bentonite was prepared by using hexadecyl trimethyl ammonium bromide (HDTMA) under ultrasonic conditions in this experiment. The modified bentonite was used to treat the acid brilliant scarlet GR and the adsorption behavior was investigated. The results indicated that the adsorption isotherm data were well fit for the Langmuir isotherm model and the pseudo two-order adsorption kinetics equation has the highest goodness of fit and the best linear correlation.

Abstract: Determination of phentolamine-bovine serum albumin (BSA) interaction based on a flow-injection microdialysis sampling fluorescence system (FI-MD-FL) was proposed. The analytical procedure was based on the oxidation of phentolamine by acidic Ce(IV) and monitoring of the fluorescence intensity of the formed Ce(III). The drug of phentolamine and BSA were mixed in different molar ratios in Ringer’s solution and incubated in a water bath. The microdialysate samples were on-line merged with acidic Ce(IV) solution by putting a microdialysis probe into the mixed solution and perfused with Ringer’s solution at 10 μL min-1. Then the sample was reached the flow cell, excited and monitored at 256/355nm (λex/λem). The dialytic efficiency under the experimental conditions was 38.8±2.2% (n=3). The data obtained was analyzed with Scatchard analysis and Klotz plot. The estimated association constant (K) and the number of the binding site (n) on one molecule of BSA by Scatchard analysis were 1.78×105 L mol-1 and 0.69, respectively. The proposed method has been applied to the study of the binding of phentolamine to bovine serum albumin (BSA) in vitro. The method provided a fast and simple technique for the study of drug-protein interactions.

Abstract: In this study the natural dye was extracted from Ginkgo leaves with the ethanol solution and then the obtained dye was applied to dye tussah silk strips with direct staining methods. The best dye conditions of the tussah silk strips could be obtained when the bath ratio was 1:50, the concentration of ginkgo pigment was 10%, the temperature of dyeing was 70°C and the time of dyeing was 60 minutes. The different colors could be obtained under different experimental conditions. The color of sample which was dyed with pigment which reacted with NaOH solution was bright and yellowish green and the color of sample which was directly dyed with pigment was orange.