Study on the Directional Breeding of High Glutamic-Acid Production Strain GTS190 and the Control on its Fermentation

Zhi Hua Li; Ning Chen

doi:10.4028/www.scientific.net/AMR.573-574.1029

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 573-574Study on the Directional Breeding of High...

Study on the Directional Breeding of High Glutamic-Acid Production Strain GTS190 and the Control on its Fermentation

Abstract:

Using Brevibacterium flavum CN1021 as starting strain, the L-glutamate producing strain was directionally bred with protoplast UV mutagenesis, UV mutagenesis and DES mutagenesis. Through shake-tube preliminary screening, shake-flask re-screening, genetic markers verification and continuous passage, an L-glutamate temperature-sensitive mutant strain GTS190 (Ts+Omr+GluHXr+ SGr+FEAr+AECr+Sucg) was screened finally. It was fermented for 36h in a vessel (30L) feeding by batches, in which glutamic acid production reached 195g/L and sugar and acid conversion rate was 66%.

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Periodical:

Advanced Materials Research (Volumes 573-574)

Pages:

1029-1033

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.573-574.1029

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Online since:

October 2012

Authors:

Zhi Hua Li, Ning Chen

Keywords:

Fermentation, L-Glutamic Acid, Protoplast, Temperature-Sensitive Mutant Strain

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References

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