Design of Automatic Nucleic Acid Extraction Instrument Based on Magnetic Nanobeads

Abstract:

In this paper, an automatic nucleic acid extraction instrument is described. It consists of mechanical components, motor controlling module, temperature controlling module and computer. Also, a prototype has been designed and constructed. The maximum sample throughput of this prototype is 96, the sample volume is 300-1000ul, the maximum sample handling time is about 60min, the sample temperature controlling range is from room temperature to 95oC, and the accuracy of temperature controlling achieved is about ± 2oC.

• Periodical: Advanced Materials Research (Volume 621)
• Pages: 313-316
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.621.313
• Online since: December 2012
• Authors: Yong Jie Dou, Jian Sheng Xu, Cong Liang Deng, Xiao Li Zhao, Leng Nie, Yang Yu, Ming Wang, Tao Song
• Keywords: Magnetic Bead Separation, Magnetic Nanobeads, Nucleic Acid Extraction

References

[1] Yongliang Cheng. The progress of the nucleic acid extraction methods. QIAGEN biological.

[2] Sonja Berensmeier. Magnetic particles for the separation and purification of nucleic acids, J. Appl Microbiol Biotechnol. 73(2006) 495–504.

DOI: 10.1007/s00253-006-0675-0

[3] Hawkins TL, McKernan KJ, Jacotot LB, et al. A magnetic attraction to high-throughput genomics, J. Science. 276(1997) 1887 ~ 1889.

DOI: 10.1126/science.276.5320.1887

[4] Stefano R, Vago L. Use of magnetic beads for tissue DNA extraction and IS6110 Mycobacterium tuberculosis PCR, J. Mol Pathol. 52 (1999) 158-160.

DOI: 10.1136/mp.52.3.158

[5] Flagstad O, Roed K, Stacy JE, et al．Reliable noninvasive genoty-ping based on excremental PCR of nuclear DNA purified with a magnetic bead protocol, J. M0l Ecol. 8(1999) 879-883.

DOI: 10.1046/j.1365-294x.1999.00623.x

[6] Jürgen Oster, Jeffrey Parker, Lotharà Brassard. Polyvinyl-alcohol-based magnetic beads for rapid and efficient separation of specific or unspecific nucleic acid sequences, J. Journal of Magnetism and Magnetic Materials. 225(2001) 145-150.

DOI: 10.1016/s0304-8853(00)01243-9

[7] Melanie Störmer, Knut Kleesiek, Jens Dreier. High-Volume Extraction of Nucleic Acids by Magnetic Bead Technology for Ultrasensitive Detection of Bacteria in Blood Components, J. Clinical Chemistry. 53(2007) 104-110.

DOI: 10.1373/clinchem.2006.070987

[8] Sorasak Intorasoot, Rujira Srirung, Amornrat Intorasoot, Surachai Ngamratanapaiboon. Application of gelatin-coated magnetic particles for isolation of genomic DNA from bacterial cells, J. Analytical Biochemistry. 386(2009) 291–292.

DOI: 10.1016/j.ab.2008.12.032

[9] Cui Ya-li，Li Zheng，Fang Yu．The application of magnetic particles in the Nucleic Acids Research, J. Journal of Northwestern Agricultural University. 28(2000) 80-85.

[10] Safarik I, Ptackova L, Safarikova M. Large-scale separation of magnetic bioaffinity adsorbents, J. Biotechnol Lett. 23(2001) 1953–(1956).

[11] Franzreb M, Siemann-Herzberg M, Hobley TJ, Thomas ORT. Protein purification using magnetic adsorbent particles, J. Appl Microbiol Biotechnol. 70(2006) 505-516.

DOI: 10.1007/s00253-006-0344-3

[12] M. Nagy, P. Otremba, C. Kru¨ger, S. Bergner-Greiner, P. Anders, B. Henske, M. Prinz, L. Roewer. Optimization and validation of a fully automated silica-coated magnetic beads purification technology in forensics, J. Forensic Science International. 152(2005).

DOI: 10.1016/j.forsciint.2005.02.027

[13] Centre for Evidence-based Purchasing. Automated nucleic acid extraction systems( 2009).

[14] Information on http: /b. jcheng. net/down/47. html.