Bacillus megaterium sp. as Degradation Agent for Biodegradable Natural Rubber Latex Films

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Degradation for biodegradable natural rubber (NRL) films by Bacillus megaterium sp. is investigated. A common soil bacterium, Bacillus megaterium sp. is one of the largest eubacteria. This bacterium has large potential in degradation process for biodegradable natural rubber latex film. Specified method is used to cultivate the bacteria using suitable media. The growth of bacteria is studied through optical density and dry weight as a function of time. The optical density at specific wavelength with different stages of bacterial growth is used to determine their mid-log growth phase of the cell. The optimum time achieved to insert NRL films is between 17 to 20 hours of incubation period. Growth curve of Bacillus megaterium sp. gives an equation of Y=2.3401X. The biodegradation behaviour of Bacillus megaterium sp. is further investigated to access the viability as accelerating media for biodegradation of NRL films.

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813-817

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December 2012

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© 2013 Trans Tech Publications Ltd. All Rights Reserved

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[1] A.J. F Carvalhoa, A. E Jobb, N Alvesb, A.A. S Curveloa, A Gandin: Thermoplastic starch/natural rubber blends, Carbohydrate Polymers Vol. 53 (2003), p.95–99.

DOI: 10.1016/s0144-8617(03)00005-5

Google Scholar

[2] Rouilly, Antoine and Rigal, Luc and Gilbert, Robert: Synthesis and properties of composites of starch and chemically modified natural rubber, Polymer Vol. 45 (2004), pp.7813-7820. ISSN 0032- 3861.

DOI: 10.1016/j.polymer.2004.09.043

Google Scholar

[3] P. Apusraporn and S. Waranyou: Preparation and some mechanical properties of composite materials made from sawdust, cassava starch and natural rubber latex, World Academy of Science, Engineering and Technology 72 (2010).

Google Scholar

[4] A.R. Azura, M.M. Afiq and A.H. Izmar: Mechanical and biodegradation properties of sago starch natural rubber latex composites, School of Materials and Mineral Resources Engineering, Engineering Campus, Universiti Sains Malaysia, Penang, Malaysia (2010).

DOI: 10.30880/ijie.2019.11.02.008

Google Scholar

[5] A.S. Ameer: Role of Microorganisms in biodegradation of plastic, Department of Microbiology, Quaid-i-Azam University, Islamabad (2007).

Google Scholar

[6] K.K. Santosh, S.K. Chakrabarty, R.M. Sudhakara: Characterization of pentachlorophenol degrading Bacillus strains from secondary pulp-and-paper-industry sludge, International Biodeterioration & Biodegradation 64 (2010) pp.609-613.

DOI: 10.1016/j.ibiod.2010.05.017

Google Scholar

[7] A. Tripathi and S.K. Srivastava: Biodegradation of orange G by a novel isolated bacterial strain Bacillus megaterium ITBHU01 using response surface methodology, African Journal of Biotechnology Vol. 11(7) (2012), pp.1768-1781.

DOI: 10.5897/ajb11.2560

Google Scholar

[8] T. P Geun, J.S. Hong: Keratinolytic activity of Bacillus megaterium F7-1, a feather-degrading mesophilic bacterium, Microbiological Research 164 (2009) pp.478-485.

DOI: 10.1016/j.micres.2007.02.004

Google Scholar

[9] Y.F. Xie et. all: Degradation of dichloroaniline isomers by a newly isolated strain, Bacillus megaterium IMT21, Microbiology (2011), 157, p.721–726.

DOI: 10.1099/mic.0.045393-0

Google Scholar

[10] K.A. Jahir: Biodegradation of Azo Dye by Moderately Halotolerant Bacillus megaterium and Study of Enzyme Azoreductase Involved in Degradation, Advanced Biotech. Vol. 10 Issue 07 l (2011) pp.21-27.

Google Scholar

[11] M. Gurulakshmi, D.N.P. Sudarmani and R. Venba: Biodegradation of leather acid dye by Bacillus subtilis, Advanced Biotech (2008) pp.12-18.

Google Scholar

[12] T. Rengathavasi, J. Singaram, M.B. Ibrahim: Effect of biosurfactant and fertilizer on biodegradation of crude oil by marine isolates of Bacillus megaterium, Corynebacterium kutscheri and Pseudomonas aeruginosa, Bioresource Technology 102 (2011).

DOI: 10.1016/j.biortech.2010.08.099

Google Scholar

[13] S. Cook, P.E.F. Cudby, R.T. Davies, and M.D. Morrisy: The microstructure of natural rubber latex films (1996), Tun Abdul Razak Research Centre, Brickendonbury, Hertford, SG13 8NL, UK.

Google Scholar

[14] F.B. David: Escherichia coli grown on minimal salts versus complex media (2001), Professor of Biology and Chemistry, University of Cincinnati Clermont College, Batavia OH 45103.

Google Scholar

[15] F. Widdel: Theory and measurement of bacterial growth, Grundpraktikum Mikrobiologie, 4. Sem. (B. Sc. ) Universität Bremen.

Google Scholar