Inhibitory Kinetics of Isooctyl 4-Hydroxy-3-Methoxycinnamate on Tyrosinase-Catalyzing Reaction

Abstract:

The inhibitory kinetics of isooctyl 4-hydroxy-3-methoxycinnamate on the activity of monophenolase and diphenolase contained in tyrosinase was studied by enzymological kinetic method with Na2HPO4-NaH2PO4 buffer solution (pH=6.8) at 30 °C. Isooctyl 4-hydroxy-3-methoxycinnamate was found to inhibit the monophenolase and diphenolase activity of tyrosinase well. The isooctyl 4-hydroxy-3-methoxycinnamate concentrations leading to 50 % inhibitory rate (IC50) were 0.24 mmol/L for monophenolase and 0.45 mmol/L for diphenolase, much less than that of arbutin (IC50 =5.3 mmol/L for diphenolase activity). Isooctyl 4-hydroxy-3-methoxycinnamate could extend the lag time of tyrosinase for oxidation of L-tyrosine, 0.4 mmol/L of isooctyl 4-hydroxy-3-methoxycinnamate resulted in the extension of lag time from 1.1 min to 3.6 min. The inhibition kinetics of isooctyl 4-hydroxy-3-methoxycinnamate analyzed by Lineweaver-Burk plots demonstrated a competitive inhibitor for the oxidation of L-DOPA, the apparent Michaelis comstant, Km, and the inhibition constant for inhibitor binding with enzyme, KI, were determined to be 0.45 mmol/L and 0.20 mmol/L respectively.

• Periodical: Advanced Materials Research (Volumes 634-638)
• Pages: 655-658
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.634-638.655
• Online since: January 2013
• Authors: Sheng Zhao Gong, Xiao Xi Tai, Yun Er Yang
• Keywords: Inhibitory Effect, Isooctyl 4-Hydroxy-3-Methoxycinnamate, Kinetic, Tyrosinase

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