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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 634-638Immobilization and Thermostability...

Immobilization and Thermostability Characterization of Cephalosporin C Acylase

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Abstract:

Some kinds of epoxy supports, LX1000-EP(C), LX1000-EP(D), LKZ-116, LKZ-118 and LKZ-126 were utilized to covalently immobilize cephalosporin C (CPC) acylase, the key enzyme in the one-step enzymatic process of 7-aminocephalosporanic acid (7-ACA) production. After preliminary carrier screening, the immobilized CPC acylase with LKZ-118 as the support shows the highest activity (115 U/g) suggesting its potential application in industrial 7-ACA production. The conditions of CPC acylase immobilized on LKZ-118 to achieve higher activity and thermostability of the immobilized enzyme were optimized by adjusting pH value, buffer concentration, enzyme dosage and immobilized time. The activity of immobilized enzyme was found to be optimal at pH 8.5, in 0.85 M sodium phosphate buffer when the enzyme dosage was 500 U/g and immobilization time was 28 h.

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Periodical:

Advanced Materials Research (Volumes 634-638)

Pages:

682-688

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.634-638.682

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Online since:

January 2013

Authors:

Kai Hua Han, Hui Luo, Yao Zhen Xie, Shun Yao, Yan Hong Chang, Hui Min Yu, Qiang Li, Zhong Yao Shen

Keywords:

Cephalosporin C Acylase, Epoxy Support, Immobilisation, Thermostability

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© 2013 Trans Tech Publications Ltd. All Rights Reserved

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