Expressing Xylanases in Escherichia Coli by Cell Surface Display

Abstract:

In this research, xylan was utilized by a recombinant whole cell biocatalyst, which was developed by expressing three xylanases — β-xylosidase, endoxylanase, and α-arabinofuranosidase — on the surface of the E. coli BL21 (DE3). The xylanases were displayed on the surface of the cells by fusing with anchor proteins, Blc. The assimilation of xylan by cell surface display was the first step in the consolidated bioprocessing (CBP). This result shows that the engineering strains could be endowed with the ability to assimilate xylan. The co-display engineering strains utilized xylan and expressed less metabolic burden than the engineering strains secreting extracellular xylanases.

• Periodical: Advanced Materials Research (Volumes 634-638)
• Pages: 965-969
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.634-638.965
• Online since: January 2013
• Authors: Mei Na Zhao, Zongbao Zheng, Tao Chen
• Keywords: Cell Surface Display, Consolidated Bioprocessing, Escherichia coli, Xylan, Xylanases

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