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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 641-642Researches on Inducing Effects of Autoclavable...

Researches on Inducing Effects of Autoclavable IPTG and Lactose Solution on Gene Expression in Genetically Engineered Bacteria

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Abstract:

The inducing effects of autoclavable IPTG and lactose solution on gene expression in genetically engineered bacteria were studied. Compared with the filter-sterilized one, the autoclavable IPTG and lactose solution of the same concentration can also show the same inducing effects respectively . All these mean that the IPTG and lactose solution used to induce gene expression in genetically engineered bacteria can be prepared more simply and more convenient so that the waste produced in the process of filter-sterilization can be prevented.

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Biotechnology, Chemical and Materials Engineering II

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Periodical:

Advanced Materials Research (Volumes 641-642)

Pages:

789-792

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.641-642.789

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Online since:

January 2013

Authors:

Gan Zhao, Lin Lin Liang, Dan Yun Xu, Li Hua Liang, Dao Zhou Wang, Hai Yin Li

Keywords:

Autoclavable, Gene Expression, Genetically Engineered Bacteria, Induced Effects, IPTG, Lactose

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© 2013 Trans Tech Publications Ltd. All Rights Reserved

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[1] J. Sambrook and D. W. Russell : Molecular Cloning : A Laboratory Manual ( 3rd ed , Cold Spring Harbor Laboratory Press , 2001).

[2] Information on http: /www. novagen. com.

[3] C. Zhai，Y. Tang and Z. Gu: Chinese Journal of Veterinary Science． Vol. 17 , No. 5(1997), pp.470-472.

[4] S. Hou, X. Li and H. Ma: J. Wuhan Univ. (Natural Science Edition)． Vol. 44, No. 4 (1998）, pp.513-516.

[5] L. Chen, S. Ren, M. Xu and G. Sun : Microbiology. Vol. 36, No. 4(2009), 551-556.

[6] W. Li, J. Zhang , A. Lei and Y. Qian: Acta Agriculturae Boreali-occidentalis Sinica. Vol. 19, No. 12(2010), pp.10-16.

[7] W. Tian, X. Luo, L. Lu, M. Ni, X. Jing and T. Zhang: Chinese Journal of Biochemical Pharmaceutics . Vol. 32, No. 5(2011), 341-345.

[8] H. Shang， Y. Gu， L. Fu and T. Zhang: Chin J Biologicals . Vol. 25, No. 7(2012)，844-847.

[9] Information on http: /www. qcbio. com/iptg. htm.

[10] Information on http: /baike. baidu. com/view/571159. htm.

[11] Information on http: /www. yacoo. com. cn/techniquezones/188?locale=en_US.

[12] Information on http: /product. ch. gongchang. com/d16828229. html.

[13] Information on http: /www. chem17. com/st151778/product_1865025. html.

[14] Information on http: /baike. baidu. com/view/1058150. htm.

[15] Information on http: /hb. jdzj. com/cpview_21746774. html.

[16] Information on http: /baike. baidu. com/view/118854. htm.

[17] Information on http: /www. med66. com/new/53a270aa2011/ 2011214yuchan143858. shtml.

[18] Information on http: /baike. soso. com/v8667138. htm.

[19] Y. Zhao: Theory and Practice of Biochemical Techniques. (Wuhan University Press, Wuhan in China 1994). pp.287-321. (in Chinese).