Screening of a Mortierella isabellina Mutant for Producing Arachidonic Acid

Ming Chen; Chun Zhi Zhang; Yong Peng Yu; Guo Ren Zu; Li Chen

doi:10.4028/www.scientific.net/AMR.641-642.793

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 641-642Screening of a Mortierella isabellina Mutant for...

Screening of a Mortierella isabellina Mutant for Producing Arachidonic Acid

Abstract:

Arachidonic acid (AA) is an essential component of structural lipids in the cellular membranes of mammals. To obtain efficient AA-producing microorganism, the strain of Mortierella isabellina 3410 was subject to mutagenesis by treating the wild-type spores exposed to ultraviolet or diethyl sulfate. A mutant of M. isabellina MS-1 was screened and chosen because of its higher AA production capability. Compared with the wild strain of M. isabellina 3410, the AA production by mutant of M. isabellina MS-1 was increased about 2.5 folds. Analysis by gas chromatography (GC) indicated that AA was the main fatty acid in the lipids extracted from the dried mycelia of the mutant of M. isabellina MS-1.

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Periodical:

Advanced Materials Research (Volumes 641-642)

Pages:

793-796

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.641-642.793

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Online since:

January 2013

Authors:

Ming Chen, Chun Zhi Zhang, Yong Peng Yu, Guo Ren Zu, Li Chen

Keywords:

Arachidonic Acid, Mortierella isabellina, Mutant, Screening

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