The Effect of T-7 Peptide on Human Non-Small-Cell Carcinoma A549 Cells

Shu Jing Wang; Shan Jiang; Jia Liu; Fei Wang; Ning Chen; Lin Liu; Ying Zhao; Xiao Dan Zhang

doi:10.4028/www.scientific.net/AMR.641-642.820

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 641-642The Effect of T-7 Peptide on Human Non-Small-Cell...

The Effect of T-7 Peptide on Human Non-Small-Cell Carcinoma A549 Cells

Abstract:

7 peptide of Tumstatin(T-7 peptide) is composed of 185-191 amino acids. To study T-7 peptide antitumor activity to human non-small-cell carcinoma(A549), T-7 peptide was designed and synthesized by amino acid synthesizer. Its purity ran up to 98.45% by HPLC and MS. The effect of T-7 peptide on A549 cell growth was observed by MTT assay, growth curve and transmission electron microscopy(TEM). T-7 peptide had the effects of suppressing A549 cell growth and promoting its apoptosis, showing dose- and time-dependent. Its IC50 was 92.84 μg/ml. TEM also revealed that A549 cell treated with T-7 peptide appeared apoptotic morphology,such as cell pyknosis and mitochondrial vacuoles formed. While T-7 peptide had little effect on human umbilical vein endothelial cells(ECV304). These researches were significant to treat human non-small-cell carcinoma in the future.

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Periodical:

Advanced Materials Research (Volumes 641-642)

Pages:

820-823

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.641-642.820

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Online since:

January 2013

Authors:

Shu Jing Wang, Shan Jiang, Jia Liu, Fei Wang, Ning Chen, Lin Liu, Ying Zhao, Xiao Dan Zhang

Keywords:

Cell Apoptosis, Cell Proliferation, Non-Small-Cell Carcinoma, T-7 Peptide, Tumstatin

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References

[1] Hamano Y, Zeisberg M, Sugimoto H, et al. Physiological levels of tumstatin, a fragment of collagen IV alpha3 chain, are generated by MMP-9 proteolysis and suppress angiogenesis via alphaV beta 3 integrin[J]. CancerCell, 2003, 23(6): 589-601.

DOI: 10.3410/f.1014883.195864

Google Scholar

[2] Paul E, Carrington CS, Yu F, Wei JM. et al. The Structure of FADD and Its Mode of Interaction with Procaspase-8 [J]. Molecular Cell, 2006, 22 (5): 599-610.

DOI: 10.1016/j.molcel.2006.04.018

Google Scholar

[3] Pedraza-Farifia LG. Mechanisms of oncogenic cooperation in cancer initiation and metastasis[J]. Yale J Biol Med, 2006, 79(3-4): 95-103.

Google Scholar

[4] Patan S. Vasculogenesis and angiogenesis as mechanisms of vascular network formation, growth and remodeling[J]. J Neurooncol, 2000, 50 (12): 1-15.

Google Scholar