Simultaneous Determination of Lomefloxacin and Ciprofloxacin in Dairy Products by First-Derivative Synchronous Spectrofluorimetry

Yang Wang; Tian Tian; Lu Wang; Xiao Ya Hu

doi:10.4028/www.scientific.net/AMR.643.43

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HomeAdvanced Materials ResearchAdvanced Materials Research Vol. 643Simultaneous Determination of Lomefloxacin and...

Simultaneous Determination of Lomefloxacin and Ciprofloxacin in Dairy Products by First-Derivative Synchronous Spectrofluorimetry

Abstract:

A simple, sensitive and quick assay to simultaneously determine lomeﬂoxacin (LFLX) and ciproﬂoxacin (CPLX) had been developed by using zero-crossing first derivative constant wavelength synchronous spectrofluorimetry. Due to their similar molecule structures, it was difﬁcult to analysis and determine LFLX and CPLX simultaneously by conventional ﬂuorometry. In order to improve the sensitivity, aluminium ion was used to significantly enhance their endogenous fluorescent intensity. The different experimental parameters affecting the synchronous fluorescence intensity of the two fluoroquinolone drugs were carefully studied and optimized. The proposed method was successfully applied for the determination of the two drugs in milk samples.

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Periodical:

Advanced Materials Research (Volume 643)

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43-46

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.643.43

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Online since:

January 2013

Authors:

Yang Wang, Tian Tian, Lu Wang, Xiao Ya Hu

Keywords:

Ciprofloxacin, First-Derivative Excitation Spectrofluorimetry, Lomeﬂoxacin, Zero-Crossing Technique

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