Analysis of M Gene of Porcine Epidemic Diarrhea Virus Isolates in Different Regions of Sichuan Province

Abstract:

The highly conserved M gene of the virus PEDV that caused pigling diarrheas in 8 different regions of Sichuan was cloned and sequenced to confirm that these diarrheas were caused by virus of at least 4 different strains. Sequence analysis was then conducted to the measured gene sequence to obtain their genetic relationships with the CV777 strain and their positions in the phylogenetic tree. Bioinformatics software DNAStar Protean was utilized to predict the antigenic domain of M gene which encoded M protein of the CV777 strain. Truncation gene corresponded to major antigenic domain was selected for analysis of its secondary structure, flexible region of protein skeleton (protein backbone) and predict the surface accessibility. Online prediction of hydrophilicity of this protein and transmembrane domains were also conducted. All these were done for the purpose of finding out whether the angigenicity changes when mutation of the four cloned unused M genes occurs.

• Periodical: Advanced Materials Research (Volume 647)
• Pages: 450-455
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.647.450
• Online since: January 2013
• Authors: Jiang Mei, Yang Ze Xiao, Yao Xue Ping, Peng Bin, Liu Bo, Wu Xu Long, Wang Yin, Tsering Choezom
• Keywords: Bioinformatics, M Gene, Porcine Epidemic Diarrhea Virus

References

[1] E. Barbara,S. Straw, D. Sylvie, W.L. Menglin: Diseases of Swine, The eighth edition, Peking: China Agricultural University Press, (2000), p.181—187.

[2] M.B. Pensaert, P. A . Debouck: New Coronavirus-like Particle Associated With Diarrhea in Swine, Arch Virol, 58(1978), pp.243-247.

DOI: 10.1007/bf01317606

[3] J.F. Timoney, J.H. Gillespie F.W. Scott: Microbiology and Infectious Diease of Domestic Animals, Comstock Publishing Associates, Ithaca, (1988), p.897—898.

[4] K. Andries,M. Pensaert: Virus Isolated and Immunofluoreseence in Different Organs of Pigs Infected with Hemagglutinating Encephalomyelitis Virus, Am J Vet Res, 41(1980), p.219—223.

[5] A.D. Lema R.D. Glook W.L. Mengelng: Diseases of Swine, Iowa State University Press (1986), pp.402-406.

[6] Y.X. Ni,J.H. Li,K.W. He: Overview of Pig Epidemic Diarrhea Research, Animal Husbandry & Veterinary Medicine33(1)pp.38-44. (2011).

[7] J.F. Chen,L. Feng H.Y. Shi: Genetic Variation and Prokaryotic Expression of N Protein Gene of Porcine Epidemic Diarrhea Virus CH/S Strain, Chinese Journal of Preventive Veterinary Medicine, 29(2007), pp.856-861.

[8] A.M. Comelis, L. Kuo P.S. Masters: Coronavirus Particle Assembly: Primary Structure Requirements of the Membrane Protein, 72(1988), pp.6838-6850.

DOI: 10.1128/jvi.72.8.6838-6850.1998

[9] L.S. Sturman,N. V. Holmes: the Molecular Biology of Coronaviruses, Adv Virus Res, 28 (1983), pp.35-112.

[10] A. Bridgen,R. Kocherhans,K. Tobler: Further Analysis of the Genome of Porcine Epidemic Diarrhea Virus, Sdv Exp Med Biol, 440(1998), pp.781-786.

DOI: 10.1007/978-1-4615-5331-1_101

[11] J.H. Fan Y.J. Li,: Cloning and Sequence Analysis of the M Gene of Procine Epidemic Diarrhea Virus LJB/03, Virus Genes, 30(2005), pp.69-73.

DOI: 10.1007/s11262-004-4583-z

[12] Z.B. Zhang: Identification of Antigenic Epitopes on the Membrane Protein and Nucleocapsid Protein of Porcine Epidemic Diarrhea Virus, Chinese Academy of Agricultural Science(2011).