Enzyme-Linked Immunosorbent Assay for Streptomycin in Animal Feeds

Hui Ying Zhang; Jun Ping Wang

doi:10.4028/www.scientific.net/AMR.651.280

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Enzyme-Linked Immunosorbent Assay for Streptomycin in Animal Feeds
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HomeAdvanced Materials ResearchAdvanced Materials Research Vol. 651Enzyme-Linked Immunosorbent Assay for Streptomycin...

Enzyme-Linked Immunosorbent Assay for Streptomycin in Animal Feeds

Abstract:

Polyclonal antibody against streptomycin was prepared by using a streptomycin–bovine serum albumin conjugate for the immunization of rabbits. Using this antibody, we developed quantitative assays for streptomycin by means of an indirect competitive enzyme-linked immunosorbent assay (icELISA). Fifty percent inhibition concentration (IC₅₀) for the antibody was 3.6 ng/ml. The detection limit was 0.4 ng/ml. The average of recoveries for all samples was 86.14% and the coefficients of variation of intra- and inter-assays were below 18%. The detection limit using the kit was 15 ng/ml in animal feeds.

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Periodical:

Advanced Materials Research (Volume 651)

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280-283

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https://doi.org/10.4028/www.scientific.net/AMR.651.280

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Online since:

January 2013

Authors:

Hui Ying Zhang, Jun Ping Wang

Keywords:

Animal Feeds, Detect, ELISA, Streptomycin

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