Analysis of the Sequence Conservation of the Circadian Clock Protein KaiC

Liu Sen; Song Liu; Fei Yun Chen

doi:10.4028/www.scientific.net/AMR.699.184

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HomeAdvanced Materials ResearchAdvanced Materials Research Vol. 699Analysis of the Sequence Conservation of the...

Analysis of the Sequence Conservation of the Circadian Clock Protein KaiC

Abstract:

Regulation of daily physiological functions with approximate a 24-hour periodicity, or circadian rhythms, is a characteristic of eukaryotes. So far, cyanobacteria are only known prokaryotes reported to possess circadian rhythmicity. The circadian system in cyanobacteria comprises both a post-translational oscillator (PTO) and a transcriptional/translational feedback loop (TTFL). The PTO can be reconstituted in vitro with three purified proteins (KaiA, KaiB, and KaiC) with the existence of ATP. Phase of the nanoclockwork has been associated with the phosphorylation states of KaiC, with KaiA promoting the phosphorylation of KaiC, and KaiB de-phosphorylating KaiC. Here we studied the sequence variation of 65 KaiC proteins in evolution, and determined some key residues in KaiC by analyzing the site variation rates of the protein sequences. These key residues could be used to study the key interactions of KaiC with KaiA and KaiB.

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Advanced Materials Research (Volume 699)

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184-188

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https://doi.org/10.4028/www.scientific.net/AMR.699.184

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May 2013

Authors:

Liu Sen, Song Liu, Fei Yun Chen

Keywords:

Circadian Rhythm, Kai, KaiABC, Post-Translational Oscillator

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