Synthesis of Nimetazepam - Molecularly Imprinted Polymer and SPE Application

Abstract:

The synthesis and performance of a molecularly imprinted polymer (MIP) designed to enable the clean-up of the interferences existing in alprazolam (Alpra) drug samples is described. The MIP was synthesized using non-covalent molecularly imprinted method. Nimetazepam (Nimet), which has a similar chemical structure as the major impurities in Alpra drug samples, was used as the dummy template. Methacrylic acid was used as the monomer. Both the affinity and selectivity of the MIP for Nimet and structurally related compounds were examined through batch-rebinding studies. The specific binding amount of Nimet was determined to be 11.4 mg•g-1. The MIP was used as the sorbent in solid phase extraction (MI-SPE) and methanol was used as the washing solvent for the clean-up of Nimet in the Alpra complex samples. The effective clean-up of Nimet was achieved, with a recovery > 90% for Alpra, in the stimulated samples.

• Periodical: Advanced Materials Research (Volume 699)
• Pages: 200-206
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.699.200
• Online since: May 2013
• Authors: Ling Zhi Sun, Cheng Jun Sun, Yong Qin Fang, Xian Xiang Sun
• Keywords: Alprazolam, Molecularly Imprinted Polymer, Nimetazepam, Sample Clean-up, Solid-Phase Extraction (SPE), Synthesis

References

[1] D.J. Kupfer: Am. J. Psychiatry Vol.156(1999), p.165

[2] A.A. Salem, B.N. Barsoum, E.L. Izake: Anal.Chim.Acta Vol.498(2003), p.79

[3] S.Sadeghi, R.Takjoo, S.Haghgoo: Anal.Lett. Vol.35(2002), p.2119

[4] S.Liawruangrath, J.Makchit, B.Liawruangrath: Anal.Sci.Vol.22(2006), p.127

DOI: 10.2116/analsci.22.127

[5] Commission of Ministry of Health: Chinese Pharmacopoeia (Beijing, 2005)

[6] K.Venkteswarlu, R.K. Venisetty, N.R. Teiiu, S. Keshetty and M.G. Pai: J. Chromatoghic Sci.Vol.45(2007), p.537

[7] I.A. Darwish, H.H. Abdine, S.M. Amer and L.I.Al-Rayes: Int.J.Anal.Chem.Vol.2009(2009), p.257306

[8] G.A. Archer, L.H.S. Sternbach: J.Org.Chem.Vol.29(1964), p.231

[9] A.martin-Esbetan:J.Chromatogr.Sci. Vol.47(2009), p.254

[10] E. Caro, R.M. Marce, F. Borrull, P.A.G. Cormack and D.C. Sherrington:TrAC, Trends Anal. Chem. Vol.25 (2006), p.143.

DOI: 10.1016/j.trac.2005.05.008

[11] G.Vlatakis, L. Andersson, R.Muller, K.Mosbach: Nature Vol.361(1993), p.645

[12] R.J. Ansell, O.Ramstrom, K. Mosbach: Clinical Chem.Vol.42(1996), p.1506

[13] M.M. Ariffin, E.I. Miller, P.A.G. Cormack, R.A. Anderson: Anal.Chem.Vol.79(2007), p.256

[14] R.A. Anderson, M.M. Ariffin, P.A.G. Cormack, E.I. Miller: Forensic Sci. Int.Vol.174(2008), p.40

[15] Y.Jia ,R.Zhu,H.Chen: FENXI CESHI XUEBAO (J.Instrum. Anal.)Vol.31(2012), p.15

[16] E.C. Figueiredo, R. Sparrapan, G. B.Sanvido,M. G. Santos,M. A. Z. Arruda and M.N. Eberlin: Analyst Vol.136(2011), p.3753

DOI: 10.1039/c1an15198c

[17] Q.Su, C.Zeng, Y. Tang, D. E. Finlow and M. Cao: J.Chromatogr.Sci .Vol.50 (2012), p.608

[18] G.Theudoridis,A.Kantifes,P.Manesiotis,N.Raikos, H.Tsoukali-Papadopoulou: J.Chromatogr.A Vol.987(2003) , p.103

[19] X.X. Sun, Y.J. Qiu, A.J. Xie, Z.Y. Wang, Z.Q.Su: J. Jiangsu Polytech.University Vol.20(3) (2008), p.50